MtoZ Biolabs offers the UFMylated Peptide Enrichment Kit which is specifically designed for low-abundance UFM1-modified peptides, enhancing enrichment specificity and efficiency and supporting research on protein homeostasis and stress-related signaling pathways.
Product Overview
UFMylation is a post-translational modification characterized by low occurrence frequency, high dynamic regulation, and strong dependence on cellular stress states. These features pose distinct challenges for the isolation and enrichment of corresponding peptides from complex digested peptide mixtures:
1. Target Signal Dilution
UFMylated peptides represent only a very small fraction of the total peptide population and are frequently masked by unmodified peptides and other modified peptide signals, making direct detection difficult.
2. Structural Heterogeneity of Modified Peptides
UFMylation substrates are diverse, with modification sites widely distributed. Variations in peptide length and physicochemical properties increase the difficulty of achieving stable enrichment under uniform conditions.
3. Difficulty Distinguishing Ubiquitin-Like Modifications
UFMylation coexists with other ubiquitin-like modifications in biological samples, and insufficient selectivity can introduce non-target signals that interfere with data interpretation.
4. Strong Condition Dependence
The UFMylation modification state is highly sensitive to experimental workflows, and variations in lysis, digestion, or enrichment conditions can readily amplify batch-to-batch differences.
Advantages
✅ Modification-Specific Capture
The enrichment system is designed around the defining features of UFMylation, enabling precise enrichment of target peptides under limited target abundance conditions.
✅ Effective Background Signal Control
The UFMylated peptide enrichment kit provided by MtoZ Biolabs incorporates optimized washing and separation conditions to reduce the inclusion of non-target peptides, thereby improving enrichment purity.
✅ Suppression of Non-Target Signals
The enrichment workflow is designed to enhance discrimination against non-target ubiquitin-like peptides, resulting in more focused enrichment outcomes.
✅ Stable and Reproducible Results
The enrichment conditions have been systematically optimized to support consistency across batches, making the kit suitable for systematic UFMylation modification studies.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
