The Butyrylated Peptide Enrichment Kit launched by MtoZ Biolabs is specifically designed for butyrylated peptides, improving selective enrichment efficiency and supporting studies of epigenetic regulation and post-translational modifications.
Product Overview
Butyrylation primarily occurs on lysine residues and is commonly found in nuclear proteins and metabolism-related proteins. Its modification level is strongly regulated by cellular states, posing specific challenges for selective enrichment in complex digested peptide backgrounds:
1. Target Signals Are Easily Diluted
Butyrylated peptides constitute only a small proportion of the total peptide pool and are often masked by high-abundance unmodified peptides, making them difficult to detect effectively without enrichment.
2. Coexistence with Multiple Acylation Modifications
Butyrylation shares similar structural and physicochemical properties with acetylation, succinylation, and other acyl modifications, which can lead to the co-enrichment of non-target peptides during enrichment.
3. Complexity of Digested Peptide Systems
In samples derived from cells or tissues, peptides with similar functions and sequences are densely distributed, increasing the difficulty of downstream signal interpretation after enrichment.
4. Strong Dependence on Experimental Conditions
The binding and elution behavior of butyrylated peptides is sensitive to experimental conditions, and minor fluctuations may affect enrichment consistency.
Advantages
✅ Selective Enrichment
The enrichment system is designed based on the characteristics of butyrylation, enabling preferential capture of target peptides from complex peptide backgrounds.
✅ Effective Control of Non-Target Interference
Optimized separation and washing procedures reduce the co-enrichment of other acylated peptides and unmodified peptides, increasing signal concentration.
✅ Stable Enrichment Process
The butyrylated peptide enrichment kit from MtoZ Biolabs features systematically optimized enrichment conditions with good tolerance to operational variation, supporting comparability across batches.
✅ Seamless Compatibility with Downstream Analyses
Enriched butyrylated peptides can be directly applied to LC-MS/MS, Western blot, and immuno-enrichment validation for multiple analytical applications.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
