The Biotinylated Peptide Enrichment Kit developed by MtoZ Biolabs is specifically designed for low-abundance biotinylated peptides to achieve highly selective capture, which is helpful for marker protein tracking and interaction analysis.
Product Overview
Biotinylated peptides are typically present within highly complex digested peptide mixtures. Their modification characteristics and enrichment mechanisms impose specific challenges for selectivity and background control:
1. Low Proportion of Target Peptides
Biotinylated peptides represent only a limited fraction of total digested peptides and are easily masked by abundant unmodified peptide signals, resulting in insufficient sensitivity for direct detection.
2. High Non-Specific Binding Background
Biotin-affinity systems exhibit extremely strong binding capacity, which can lead to the co-capture of non-target peptides or contaminating molecules in complex samples, thereby reducing enrichment purity.
3. Substantial Physicochemical Diversity of Peptides
Differences in peptide length, charge, and hydrophobicity among biotinylated peptides increase the difficulty of achieving stable enrichment under uniform conditions.
4. Pronounced Matrix Interference
Digested peptides derived from cellular or tissue samples are highly complex, and residual salts, small molecules, and high-abundance peptides can interfere with enrichment performance.
Advantages
✅ High-Affinity Targeted Capture
The enrichment system is designed around the characteristics of biotin modification, enabling effective capture of biotinylated peptides from complex peptide backgrounds.
✅ Reduced Non-Specific Background
Optimized washing and cleanup procedures minimize co-enrichment of non-target peptides, improving the relative purity of biotinylated peptides.
✅ Compatible with Multiple Sample Types
The biotinylated peptide enrichment kit from MtoZ Biolabs is suitable for digested peptide samples from different sources, providing stable operating conditions and good reproducibility of enrichment results.
✅ Broad Downstream Platform Compatibility
Enriched biotinylated peptides can be directly applied to LC-MS/MS, Western blot, and immuno-enrichment validation for downstream analyses.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
