MtoZ Biolabs has launched the Fumaryllysine-Modified Peptide Enrichment Kit which is specifically designed for low-abundance fumarylated peptides, overcomes challenges associated with high background and modification discrimination, and supports analytical studies of metabolism-related post-translational modifications.
Product Overview
Fumaryllysine modification is a non-canonical post-translational modification closely associated with metabolic dysregulation and is commonly observed under conditions of fumarate accumulation. Enrichment of fumaryllysine-modified peptides from complex digested peptide mixtures therefore poses specific experimental challenges:
1. Low Modification Abundance
Fumaryllysine-modified peptides represent only a small fraction of the total peptide pool and are easily masked by high-abundance unmodified peptide signals.
2. Pronounced Physicochemical Changes
Introduction of the fumaryl group adds additional charge and alters peptide conformation, which can affect binding efficiency and elution stability under conventional enrichment conditions.
3. Interference from Metabolism-Related Modifications
Multiple metabolite-derived modified peptides often coexist in the sample, increasing the risk of nonspecific binding and compromising enrichment purity.
4. Complex Sample Background
Fumarylation is predominantly enriched in metabolism-related proteins, resulting in digested peptide mixtures with densely populated peptides of similar function and sequence, which complicates downstream data interpretation.
Advantages
✅ Modification-Specific Recognition
The enrichment system is optimized around the chemical and structural features of fumaryllysine modification, improving the selective capture of low-abundance target peptides.
✅ Background Interference Control
The matched binding and washing conditions reduce co-enrichment of unmodified peptides and other metabolism-related modified peptides, making the enrichment results more focused and reliable.
✅ High Enrichment Stability
The fumaryllysine-modified peptide enrichment kit from MtoZ Biolabs is designed with high tolerance to operational variation, supporting consistency and reproducibility across batches.
✅ Downstream Analysis Compatibility
The enriched peptides can be directly applied to LC-MS/MS, Western blotting, and related immunological validation platforms for downstream analyses.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
