The MtoZ Biolabs’ Palmitoylated Protein Enrichment Kit is specifically designed for the stable enrichment of palmitoylated proteins. The kit incorporates enzyme inhibitors to effectively suppress depalmitoylation and non-specific protein degradation, making it suitable for downstream proteomics and functional studies.
Product Overview
In practical palmitoylated protein enrichment workflows, the intrinsic physicochemical properties of palmitoylation and the complexity of biological samples often introduce multiple challenges that compromise experimental stability and data reliability. These challenges mainly include the following aspects.
1.
Modification Instability
S-palmitoylation is a reversible post-translational modification that is readily hydrolyzed by depalmitoylating enzymes during sample handling, resulting in signal loss.
2. High Background Interference
Non-specific binding and co-enriched proteins increase background signals and reduce enrichment specificity and data confidence.
3. Strong Sample Dependency
Variations in lipid composition across different sample types lead to inconsistent enrichment performance and limited workflow universality.
4. Limited Workflow Compatibility
Conventional enrichment approaches are often poorly compatible with downstream mass spectrometry and quantitative analysis, requiring repeated optimization.
Advantages
✅ Enzymatic Protection
An optimized enzyme inhibitor system effectively preserves palmitoylation sites and minimizes modification loss.
✅ High-Specificity Enrichment
Optimized capture chemistry and elution conditions reduce non-specific background signals.
✅ Stable and Reproducible Workflow
Standardized procedures improve consistency across different sample types.
✅ Strong Downstream Compatibility
Enriched products can be directly applied to LC-MS/MS-based identification and quantitative analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
