MtoZ Biolabs provides the HNE-Modified Peptide Enrichment Kit which is specifically designed for low-abundance HNE-modified peptides, enabling efficient enrichment and supporting research and analysis of lipid peroxidation-related post-translational modifications.
Product Overview
4-Hydroxynonenal (HNE)-modified peptides typically occur within complex lipid peroxidation backgrounds and are characterized by low abundance and similar physicochemical properties, which poses specific challenges for their enrichment and identification in complex samples:
1. Extremely Low Modification Abundance
HNE-modified peptides represent only a very small proportion of the total peptide pool and are often overwhelmed by high-abundance unmodified peptide signals, making direct detection difficult and limiting sensitivity.
2. Similarity to Other Modifications
The physicochemical properties of HNE modifications are similar to those of other lipid peroxidation-derived modifications, making effective discrimination difficult using conventional enrichment methods and resulting in suboptimal enrichment purity.
3. Strong Interference from Complex Backgrounds
Lipids, pigments, and other metabolites present in samples can cause significant interference, increasing the difficulty of selectively enriching target peptides.
4. Poor Enrichment Consistency
The enrichment process is susceptible to multiple influencing factors, leading to variability between experiments and reduced reproducibility.
Advantages
✅ High-Specificity Enrichment
The enrichment system is designed based on the characteristics of HNE modifications, enhancing the capture efficiency of low-abundance target peptides.
✅ Reduced Non-Specific Interference
Optimized washing and separation procedures minimize the co-enrichment of unmodified and other modified peptides, improving enrichment purity.
✅ Adaptable to Multiple Sample Sources
The HNE-modified peptide enrichment kit from MtoZ Biolabs is applicable to complex digested peptide samples from different sources, delivering stable enrichment performance and reliable reproducibility.
✅ High Enrichment Stability
Optimized enrichment and elution conditions reduce inter-batch variation and minimize batch-to-batch differences, enhancing process stability and ensuring experimental consistency.
✅ Strong Downstream Compatibility
Enriched peptides can be directly applied to liquid chromatography-tandem mass spectrometry analysis, Western blotting, immunoaffinity validation, and other analytical platforms.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
