The Methylated Peptide Enrichment Kit launched by MtoZ Biolabs enables specific capture of methylated peptides, achieving selective enrichment and detection, and supports mass spectrometry-based studies of protein methylation.
Product Overview
Protein methylation typically occurs on lysine or arginine residues and is characterized by diverse modification forms, low abundance, and minimal mass shifts. These properties pose unique challenges for the enrichment and identification of methylated peptides within complex digestion mixtures:
1. Low Modification Abundance
Methylated peptides represent only a very small fraction of total peptides and are often masked by signals from high-abundance unmodified peptides, making direct detection difficult.
2. Subtle Modification Differences
The mass differences among mono-, di-, and tri-methylation are small, placing high demands on enrichment selectivity and resolution.
3. Strong Background Peptide Interference
Non-specific binding can introduce large numbers of unmodified or other PTM peptides, reducing enrichment purity.
4. High Sample Complexity
Samples derived from cells, tissues, or body fluids have complex backgrounds, further increasing the difficulty of isolating methylated peptides.
Advantages
✅ High-Selectivity Recognition
The enrichment system is optimized for the characteristics of methylation modifications, enhancing specific capture of peptides with different methylation states.
✅ Significant Background Reduction
Optimized washing and cleanup procedures effectively reduce co-enrichment of unmodified peptides, improving the purity of methylated peptides.
✅ Compatibility with Multiple Sample Types
The methylated peptide enrichment kit from MtoZ Biolabs is suitable for digested peptide samples derived from cells, tissues, and other sources, providing stable performance and good reproducibility.
✅ Strong Downstream Platform Compatibility
The enriched methylated peptides can be used for LC-MS/MS, Western blot, and immuno-enrichment validation, supporting multiple downstream analytical approaches.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
