The Sputum Protein Extraction Kit developed by MtoZ Biolabs is specifically designed for the rapid isolation of high-quality proteins from sputum samples and overcomes multiple interferences caused by high viscosity, mucin-rich networks, and microbial impurities. This kit enables the rapid acquisition of high-purity proteins, suitable for respiratory diseases, infectious diseases, biomarker discovery, and multi-omics research.
Product Overview
Sputum is a respiratory secretion rich in proteins, inflammatory mediators, and potential biomarkers. However, its complex physicochemical properties present several challenges to protein extraction:
1. High Viscosity and Mucin Network Structure
Sputum contains abundant mucin, which forms gel-like structures that make protein release and lysis extremely difficult, significantly reducing extraction efficiency.
2. High Microbial and Cellular Debris Content
Sputum contains large amounts of bacteria, fungi, immune cells, and exfoliated epithelial cells, which can interfere with protein purity and affect subsequent analysis.
3. Proteins Are Prone to Oxidation and Enzymatic Degradation
The inflammatory environment in sputum is often accompanied by reactive oxygen species (ROS) and various proteases, which can lead to protein degradation, conformational changes, and affect the stability and function of target proteins, reducing the reliability of research outcomes.
Advantages
✅ Efficient
This kit features a unique de-viscosity formula that significantly lowers the sample viscosity and promotes complete protein release.
✅ Optimized
The optimized purification process ensures a clean protein extract that is suitable for high-sensitivity analyses, such as mass spectrometry and immunodetection.
✅ Protective
MtoZ Biolabs' Sputum Protein Extraction Kit includes antioxidants and a protease inhibitor system, preserving the structure and function of target proteins.
✅ Compatible
The extracted protein can be directly used for various high-sensitivity research applications, including: SDS-PAGE, Western blot, LC-MS/MS, BCA protein quantification, Immunoassays, and Biomarker discovery.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
