The Sperm Histone Extraction Kit from MtoZ Biolabs is specifically engineered for the efficient isolation of tightly packed histones from highly condensed sperm chromatin, delivering high extraction efficiency and reliable recovery, and supporting advanced sperm epigenetics research.
Product Overview
Sperm chromatin is extremely compact, with most histones replaced by protamines. This unique structural organization presents significant challenges for histone extraction:
1. Ultracondensed Chromatin Structure
Sperm nuclear proteins are dominated by protamines, leaving histones at very low abundance and tightly embedded. Conventional lysis methods cannot sufficiently disrupt the structure or release target histones.
2. Highly Resilient Membrane and Acrosomal Structures
The sperm plasma membrane and acrosome are exceptionally robust, and standard physical or chemical lysis is often insufficient to fully disrupt the cell, resulting in low extraction efficiency.
3. Histones Are Easily Masked by Background Proteins
Sperm contain abundant non-histone proteins that increase sample impurities after extraction, interfering with downstream SDS-PAGE, ChIP, and mass spectrometry analysis.
Advantages
✅ Optimized
The specialized lysis buffer efficiently disrupts the protamine-dominated ultracondensed chromatin, significantly enhancing histone release.
✅ Efficient
The optimized chemical extraction buffer enriches low-abundance sperm histones while reducing background interference.
✅ Compatible
Histones extracted by MtoZ Biolabs' Sperm Histone Extraction Kit are compatible with Western blot, LC-MS/MS, ChIP, and other applications.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
