The Whey Protein Extraction Kit developed by MtoZ Biolabs enables rapid and efficient protein extraction from whey samples. The kit enables the rapid recovery of high-purity soluble proteins suitable for advanced proteomic analysis.
Product Overview
Whey is the pale-yellow supernatant obtained after casein precipitation or curd formation. It consists primarily of soluble proteins, lactose, electrolytes, and small amounts of lipids. As a widely used sample type in nutritional research, functional protein studies, and milk-derived biomarker analysis, whey still presents several challenges that affect protein purity and stability:
1. Low Protein Concentration and Dilution Effect
The overall protein content of whey is considerably lower than that of whole milk, making low-abundance proteins difficult to enrich. Conventional extraction methods often fail to provide protein concentrations sufficient for mass spectrometry–based analysis.
2. High Lactose and Salt Content Interfere with Lysis and MS Analysis
Whey contains substantial amounts of lactose, electrolytes, and small metabolites that can disrupt protein binding, reduce lysis buffer stability, and cause ion suppression during mass spectrometry, ultimately compromising detection sensitivity.
3. Residual Lipids and Trace Casein Affecting Sample Clarity
Although the lipid content is much reduced compared with milk, whey still contains lipid microdroplets and dispersed casein fragments. Without efficient removal, these impurities impair electrophoretic clarity and interfere with accurate protein quantification.
4. Protein Instability Requiring Rapid Protection
Whey contains endogenous proteases and hydrolases that can degrade structural and functional proteins if not promptly inhibited, affecting the quality of functional studies and quantitative proteomics.
Advantages
✅ Optimized
MtoZ Biolabs' Whey Protein Extraction Kit features a binding-enrichment system optimized for the low-protein background of whey.
✅ Efficient
The purification procedure in the kit produces a high-purity protein sample suitable for high-sensitivity analytical techniques.
✅ Excellent Yields
A specialized clarification system yields uniform, transparent whey protein solutions ideal for precise downstream assays.
✅ Protective
The optimized protease inhibition formulation acts immediately during lysis to suppress proteolytic activity, effectively preventing whey protein degradation and ensuring reliable quantitative and proteomic outcomes.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
