The Bile Protein Extraction Kit designed by MtoZ Biolabs is a complete set of reagents for the extraction of proteins from bile samples. The kit enables reliable recovery of high-purity bile proteins, supporting research on hepatobiliary diseases and the digestive system.
Product Overview
Bile is an important biofluid for evaluating hepatobiliary function, biliary tract diseases, and tumor-associated biomarkers. It reflects hepatic secretion activity, bile acid metabolism, and the local microenvironment within the biliary system. However, the complex biochemical composition of bile poses significant challenges for protein extraction and purification:
1. Strong Detergent Activity Leading to Protein Denaturation
Bile contains high levels of bile salts with strong surfactant properties that can rapidly disrupt protein structures. This makes it difficult for conventional lysis systems to maintain protein conformation and detectability, ultimately affecting the quality of downstream analyses.
2. High Salt and Lipid Content Interfere with Extraction
The elevated concentrations of salts, cholesterol, and lipids in bile can destabilize lysis buffers, reduce protein binding efficiency, and cause ion suppression during mass spectrometry, thereby decreasing protein detection sensitivity.
3. Variable Viscosity and Interference from Cellular Debris
Bile viscosity fluctuates depending on health status and sampling location. Some samples may contain large amounts of epithelial cells, inflammatory cells, or crystalline deposits, increasing the difficulty of lysis and compromising the clarity of protein-containing supernatants.
Advantages
✅ Gentle
The kit helps maintain protein complexes for reliable immunoassays and mass spectrometry analysis.
✅ Optimized
Efficient extraction of total bile protein, including membrane-associated and low-abundance proteins within the complex bile matrix.
✅ Protective
MtoZ Biolabs' Bile Protein Extraction Kit provides a fast-acting inhibitor system that protects degradation-prone proteins from the earliest step of processing.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
