The Breast Milk Protein Extraction Kit developed by MtoZ Biolabs enables rapid and efficient protein extraction from breast milk samples. The kit ensures reliable recovery of high-purity proteins, supporting breast milk biomarker discovery, functional protein studies, and nutritional research.
Product Overview
Milk is an extremely complex natural emulsion composed of lipids, casein, whey proteins, lactose, and cellular components. It is widely used in nutrition studies, immunology research, and human milk biomarker discovery. However, the unique physical and chemical properties of milk create significant difficulties during protein extraction:
1. High Fat Content Causes Emulsification Interference
The large number of milk fat globules forms a highly stable emulsion that encapsulates proteins and prevents efficient interaction between lysis reagents and target proteins. This leads to reduced protein solubility and significantly limits extraction efficiency.
2. Casein Aggregation Affecting Clarification and Separation
Casein easily forms gel-like precipitates under certain conditions, resulting in viscous, difficult-to-clarify samples. These aggregates interfere with protein purification and compromise the accuracy of downstream analyses.
3. Complex Impurity Background Impacting Analytical Performance
Milk contains various carbohydrates, lipids, small-molecule metabolites, and cell-derived impurities. Without adequate removal, these contaminants affect mass spectrometry identification, electrophoretic resolution, and the reproducibility of immunoassays.
4. Protease Activity Leading to Target Degradation
Milk contains multiple endogenous enzymes that can rapidly degrade functional proteins, immunoglobulins, and other key biomolecules if not promptly inhibited during sample processing.
Advantages
✅ Efficient
A unique sedimentation-separation system effectively separates target proteins from casein aggregates, reducing sample viscosity and minimizing particulate interference.
✅ Compatible
The resulting protein samples are suitable for mass spectrometry, immunoassays, and quantitative protein analysis.
✅ Protective
MtoZ Biolabs' Breast Milk Protein Extraction Kit incorporates a multi-component protease inhibition system that quickly suppresses degradation reactions at the start of processing.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
