Target Information
| Target information | Nuclear factor kappa B subunit 1 (NFKB1) is produced as the p105 precursor and processed to generate the p50 NF-kappa-B subunit. NFKB1 participates in NF-kappa-B transcriptional regulation through homo- or heterodimeric complexes with other Rel family proteins, including RelA/p65. It is widely studied in inflammation, immunity, cell survival, differentiation, tumor biology, apoptosis, and stimulus-induced transcriptional activation. The p105 precursor can also contribute to cytoplasmic retention and regulation of NF-kappa-B proteins, while p50 functions as a DNA-binding subunit after processing. MtoZ Biolabs provides IP-MS Validated NFKB1 p105/p50 Polyclonal Antibody for enrichment of NFKB1 p105 and p50-containing samples before downstream mass spectrometry analysis. This NFKB1 p105/p50 antibody validated by IP-MS supports immunoprecipitation-based preparation of NFKB1 protein material in NF-kappa-B pathway proteomics workflows. It can be used in studies requiring an NFKB1 p105/p50 IP antibody or NFKB1 p105/p50 antibody for immunoprecipitation, with interpretation considering precursor and processed subunit forms. |
|---|---|
| Target name | nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 |
| Aliases | NFKB1, DNA binding factor KBF1, DNA-binding factor KBF1, EBP 1, EBP-1 |
| UniProt accession | P19838 |
Product Introduction
| Primary applications |
Before delivery, each antibody undergoes IP-MS validation to verify its ability to recognize and enrich the intended target protein |
|---|---|
| Other applications | WB, IHC, IF, ICC, FC (Intra), ELISA, IF, ChIP, RIP |
| Species reactivity | Human, Rat, Mouse, Pig, Rabbit, Canine, Monkey, Chicken, Bovine, Duck |
| Clonality | Polyclonal |
| Host | Rabbit |
| Recommended dilution ratio | IP: 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Specification | 50 μL,100 μL,150 μL |
| Storage condition | Short-term storage at 2-8 °C. Long-term storage at -20 °C. Avoid repeated freeze-thaw cycles |
MtoZ Biolabs IP-MS Workflow Products
MtoZ Biolabs offers a fully integrated IP-MS workflow solution, combining antibodies validated for IP-MS with expertly paired IP kits and seamless downstream mass spectrometry services. Our platform empowers researchers to achieve reliable target enrichment, protein interaction mapping, and comprehensive proteomic profiling with confidence and efficiency.
IP-MS Validated Antibodies
- Antibodies rigorously tested for IP-MS applications
- Minimize experimental uncertainty and accelerate research timelines
- Broad applicability across multiple species and protein targets
Expertly Paired IP Kits
- Designed and validated to complement MtoZ IP-MS antibodies
- Detailed step-by-step experimental protocols for reproducible target enrichment
- Optimized workflows to maximize antibody performance
Comprehensive IP-MS Analysis Services
- Submit immunoprecipitated samples directly to MtoZ Biolabs
- High-resolution mass spectrometry for target verification, interaction mapping, and proteomics studies
- Structured, publication-ready reports for seamless documentation and data reproducibility
Antibody Validation Levels
To support different research goals, budgets, and evidence requirements, our antibodies are available with multiple validation levels. Each antibody undergoes IP-MS validation before delivery to confirm target-specific recognition and enrichment. For projects requiring additional confirmation, Western blot validation can be added to provide orthogonal evidence of target detection.
| Validation level | Included validation | Best for |
|---|---|---|
| IP-MS Only | Confirms target-specific enrichment by immunoprecipitation followed by mass spectrometry analysis. | IP-based protein enrichment and target specificity assessment. |
| Western Blot Only | Confirms target detection by Western blot under defined experimental conditions. | Routine target detection and expression-level assessment. |
| IP-MS + Western Blot | Combines mass spectrometry-based target enrichment evidence with Western blot-based detection evidence. | Customers requiring more comprehensive validation support. |
Antibody Validation Workflow
Validation Report Included
IP-MS
- IP experiment record
- Antibody usage information
- Original experimental image
- Mass spectrometry parameters
- Identified target peptides
- Representative MS/MS spectra
Western Blot
- WB experimental conditions
- Original blot image
- Qualitative target band recognition result
MtoZ Biolabs IP-MS Antibody Solutions
Traditional Antibody Supplier
Conventional workflowCustomers need to purchase small amounts of antibody for in-house testing
01Validation depends on customer-provided samples and workflows
02Suppliers usually stop support after product delivery
03Customers must find a separate provider for MS or interaction analysis
04Higher risk of repeated trial-and-error
05
MtoZ Biolabs IP-MS Antibody Solutions
RecommendedMtoZ Biolabs can provide IP-MS validation before or at the time of purchase
01Validation is supported by MS-based evidence and a detailed report
02Antibody purchase can be directly connected to downstream IP-MS services
03Antibody and downstream IP-MS analysis can be completed within a single workflow
04Reduces trial attempts and accelerates project progress
05
Why Choose MtoZ Biolabs?
Antibodies Validated
Target Proteins
Research Institutions Served
Full Validation Reports Included
Structured IP/Co-IP/IP-MS validation reports are included with every antibody for easy lab recordkeeping and project documentation.
Ultra-High Resolution MS Platform
IP-MS validation on high-resolution LC-MS/MS instrumentation for confident target enrichment and specificity assessment.
