Peptide-Peptide/Protein Interaction Analysis Service
- Screening and Preliminary Validation of Peptide-Peptide/Protein Interactions (e.g., Pull-Down assays, Co-IP, ELISA)
- Affinity Measurement and Kinetic Parameter Analysis (e.g., SPR, BLI, ITC, MST)
- Interaction Site Identification and Conformational Mapping (e.g., XL-MS, HDX-MS)
- Quantitative Analysis and Interaction Strength Comparison (e.g., Label-Free or TMT-based quantitative mass spectrometry)
- Antitumor Peptide Drug Screening
- Signal Pathway Regulation Studies
- Protein Complex Structure Studies
- Virus-Host Interaction Research
- Autoimmune Disease Mechanism Investigation
Peptide-Peptide/Protein Interaction Analysis Service is designed to identify and validate binding events between specific peptides and target peptides or proteins using multi-platform technologies. This service encompasses peptide screening, affinity measurement, binding site mapping, and kinetic parameter evaluation, supporting both structural-functional research and the discovery and optimization of lead compounds.
Within the complex molecular networks of cells, peptides function not only as structural units of proteins but also as independent bioactive molecules involved in various biological processes, including signal transduction, receptor activation, protein complex assembly, and transcriptional regulation. Peptide-peptide and peptide-protein interactions play a crucial role in mediating these life activities, characterized by high specificity and spatiotemporal precision. Therefore, systematically analyzing these interactions provides key insights into underlying biological mechanisms and holds significant relevance for functional peptide drug development, vaccine design, and targeted therapies.
Using a combination of biophysical, biochemical, and structural techniques, Peptide-Peptide/Protein Interaction Analysis focuses on elucidating the binding relationship, affinity, kinetic parameters, and structural interfaces between short peptides and their target proteins or peptide partners. This analysis is of great value for functional peptide screening, epitope mapping, protein interaction network construction, and rational drug design.
Services at MtoZ Biolabs
Utilizing high-sensitivity mass spectrometry platforms and various biophysical and chemical analysis technologies, MtoZ Biolabs offers Peptide-Peptide/Protein Interaction Analysis Service to systematically characterize the binding spectrum, kinetics, and structural basis of peptide interactions with paired proteins or peptides. This service delivers accurate data to support drug discovery, signaling pathway analysis, and functional peptide development. Covering the full workflow from screening to validation and mechanistic analysis, our service includes but is not limited to:
These service modules can be flexibly combined based on specific research objectives and are fully customizable to support functional peptide drug development, vaccine candidate design, and protein function studies.
Kannan R. et al. PLOS ONE. 2015.
Figure 1. Identification of the interaction sites in protein complexes by isotope-labeled cross-linking and mass spectrometry.
Service Advantages
1. Advanced Analysis Platform: MtoZ Biolabs established an advanced Peptide-Peptide/Protein Interaction Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.
2. One-Time-Charge: Our pricing is transparent, no hidden fees or additional costs.
3. Support for Diverse Interaction Modes: Compatible with various peptide structures-including linear peptides, cyclic peptides, and modified peptides-for interaction analysis with different targets such as native or recombinant proteins.
4. Customized Service Solutions: Based on the customer's research goals, we provide modular analysis strategies to meet multi-level needs from basic research to drug development.
Sample Submission Suggestions
Sample Types: Supports synthetic peptides, natural peptides, modified peptides, recombinant proteins, and native extracted proteins.
Storage and Shipping: Peptide or protein samples should be submitted in lyophilized or solution form, avoiding interfering components such as high salt or glycerol. Samples should be stored at −80 °C and shipped using dry ice or insulated cold packs to maintain sample activity and stability.
Additional Notes: We recommend contacting us prior to sample submission to receive detailed and tailored sample preparation guidelines.
Applications
Below are examples of how Peptide-Peptide/Protein Interaction Analysis Service is applied across various research and application fields:
Screen functional peptides that specifically bind to cancer-associated proteins to assess their antitumor potential and support the development of peptide-based anticancer therapeutics.
Analyze interactions between regulatory peptides and signaling proteins to uncover their roles in the regulation of cell proliferation, differentiation, and apoptosis.
Investigate peptide-mediated binding interfaces within protein complexes to better understand the molecular basis of complex assembly and stability.
Study the binding between virus-derived peptides and host proteins to identify potential targets for antiviral drug or vaccine development.
Examine the binding between self-antigen peptides and immune-related proteins to explore possible autoimmune activation mechanisms and identify diagnostic biomarkers.
Related Services
Protein Interaction Analysis Service
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