The Whole Blood Protein Extraction Kit from MtoZ Biolabs is designed for efficient extraction of total proteins from whole blood samples, enabling high-quality protein preparations suitable for functional studies and proteomics-based analysis.
Product Overview
Protein extraction from whole blood is technically demanding due to its complex composition and high abundance of interfering components. Common challenges include:
1. Dominance of High-Abundance Proteins
Whole blood contains large amounts of hemoglobin and plasma proteins, which can mask lower-abundance proteins and reduce analytical sensitivity in downstream workflows.
2. Cellular Heterogeneity
Whole blood is a mixture of erythrocytes, leukocytes, platelets, and plasma, each with distinct biochemical properties. Non-selective extraction can lead to inconsistent protein recovery across different cellular compartments.
3. Interference from Lipids and Small Molecules
Lipids, metabolites, and anticoagulants present in blood samples may co-extract with proteins, affecting protein stability and downstream assay performance.
4. Protease Activity After Lysis
Proteases released from blood cells upon lysis can rapidly degrade proteins, compromising sample integrity and reproducibility.
Advantages
The MtoZ Biolabs Whole Blood Protein Extraction Kit provides the following benefits:
✅ Efficient Recovery from Complex Blood Matrices
The optimized extraction workflow enables reliable protein recovery from whole blood while accommodating its high cellular and protein complexity.
✅ Improved Representation of Low-Abundance Proteins
The formulation reduces interference from highly abundant blood proteins, supporting improved detection of lower-abundance targets.
✅ Consistent Extraction Across Blood Components
The kit supports uniform protein extraction from erythrocytes, leukocytes, and platelets, improving reproducibility between samples.
✅ Protection Against Protein Degradation
Integrated inhibitor systems limit protease-mediated degradation during extraction, preserving protein integrity for downstream analysis.
✅ Compatibility with Functional and Proteomics-Based Workflows
Extracted proteins are suitable for biochemical assays, immunoblotting, and mass spectrometry-based proteomic profiling.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
