The Total Protein Extraction Kit from MtoZ Biolabs is specifically engineered for blood-derived samples, enabling highly efficient recovery of comprehensive protein profiles while eliminating phospholipid and salt interference, thereby establishing a clean and reliable starting point for downstream analytical workflows.
Product Overview
Serum and plasma represent invaluable biomarker sources; however, their inherent complexity poses substantial challenges to total protein extraction:
1. Masking by High-Abundance Proteins
Albumin and immunoglobulins constitute over 90% of the total protein content, which severely obscures the detection signals of low-abundance protein species.
2. Interference from Phospholipids and Lipids
Lipoproteins and phospholipids can form micelles or co-precipitate with proteins during extraction, reducing yield and causing pronounced ion suppression in mass spectrometry analyses.
3. Residual Salts and Metabolites
Incomplete removal of salts and small metabolites in serum/plasma directly compromises protein quantification accuracy and may damage downstream instrumentation.
Advantages
✅ Efficient Recovery with Broad Compatibility
The MtoZ Biolabs Total Protein Extraction Kit employs optimized lysis chemistry that effectively disrupts protein–protein interactions, thereby enabling high-efficiency recovery of diverse proteomic components, including low-abundance proteins, while ensuring full compatibility with downstream Western blotting, ELISA, and mass spectrometry applications.
✅ Effective De-Lipidation and Desalting
Proprietary purification procedures efficiently eliminate phospholipids, lipids, and salts, producing clean protein preparations that markedly enhance mass spectrometric sensitivity and accuracy.
✅ Fast Workflow with Minimal Sample Loss
A streamlined spin-column protocol is completed within 1 hour, minimizing protein loss and making the kit particularly suited for precious low-volume clinical specimens.
✅ Preservation of Protein Integrity
Mild formulation conditions maintain protein structural stability and prevent degradation of target proteins throughout extraction.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
