The Platelet Mitochondrial Protein Extraction Kit from MtoZ Biolabs is designed for efficient isolation of mitochondrial proteins from platelets, enabling high-purity mitochondrial preparations for mitochondrial functional studies and proteomics-based analysis.
Product Overview
Extracting mitochondrial proteins from platelets presents distinct technical challenges due to the unique biological and structural properties of platelets:
1. Limited Starting Material
Platelets are small, anucleate cells with limited biomass. Inefficient extraction can lead to insufficient mitochondrial protein yield for reliable downstream analysis.
2. Sensitivity to Platelet Activation
Platelets are highly sensitive to mechanical stress and chemical stimuli. Uncontrolled handling or lysis conditions may induce activation-associated changes that alter mitochondrial protein composition.
3. Difficulty in Mitochondrial Enrichment
Mitochondrial proteins can be co-extracted with cytosolic or membrane-associated proteins, reducing mitochondrial specificity and complicating interpretation of functional data.
4. Structural Fragility of Platelet Mitochondria
Platelet mitochondria are particularly sensitive to harsh detergents and excessive mechanical force, which can compromise membrane integrity and result in protein loss.
Advantages
The MtoZ Biolabs Platelet Mitochondrial Protein Extraction Kit provides the following benefits:
✅ Efficient Recovery from Low-Input Samples
The optimized extraction workflow supports reliable mitochondrial protein recovery from limited platelet material.
✅ Preservation of Mitochondrial Integrity
Carefully controlled extraction conditions help maintain mitochondrial structure, supporting accurate functional characterization.
✅ Improved Mitochondrial Specificity
The extraction strategy minimizes co-extraction of non-mitochondrial proteins, yielding cleaner mitochondrial protein preparations.
✅ Protection Against Protein Degradation
Integrated inhibitor systems reduce protease-mediated degradation during extraction, preserving protein integrity.
✅ Compatibility with Functional and Proteomics-Based Workflows
Extracted mitochondrial proteins are suitable for mitochondrial functional assays, immunoblotting, and mass spectrometry-based proteomic profiling.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
