The Peripheral Blood Mononuclear Cell (PBMC) Protein Extraction Kit from MtoZ Biolabs is designed for efficient extraction of high-quality total protein from PBMC samples, delivering clean and reproducible protein lysates suitable for immunology and proteomics workflows.
Product Overview
PBMCs, including lymphocytes and monocytes, are widely used in immunological and translational research. However, protein extraction from PBMC samples presents several technical challenges:
1. Limited Sample Quantity
PBMCs are often obtained in small numbers from peripheral blood, requiring extraction methods that maximize protein recovery from low-input samples.
2. Cellular Heterogeneity
PBMC populations consist of multiple immune cell subtypes with distinct protein expression profiles, making consistent and representative protein extraction essential.
3. Sensitivity to Extraction Conditions
Immune cell proteins, particularly signaling and regulatory proteins, are sensitive to harsh lysis conditions that can lead to degradation or loss of functional integrity.
4. Endogenous Protease Activity
Proteases released during cell lysis can rapidly degrade target proteins, affecting data quality and reproducibility in downstream analyses.
Advantages
The MtoZ Biolabs Peripheral Blood Mononuclear Cell (PBMC) Protein Extraction Kit provides the following benefits:
✅ High Recovery from Low Cell Numbers
The optimized lysis system enables efficient protein extraction from limited PBMC inputs while maintaining consistent yield.
✅ Uniform Lysis Across PBMC Subtypes
The formulation supports effective disruption of diverse immune cell types, ensuring representative protein profiles from heterogeneous PBMC populations.
✅ Protection of Protein Integrity
Integrated protease inhibition minimizes protein degradation during extraction, preserving protein integrity for downstream analysis.
✅ Low Background and High Purity
The extraction process yields clean protein lysates with minimal interference, supporting sensitive analytical techniques.
✅ Broad Downstream Compatibility
Extracted proteins are suitable for Western blotting, ELISA, LC-MS/MS-based proteomics, and immune signaling studies.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
