The Polyacrylamide Spin Desalting Columns from MtoZ Biolabs are engineered for rapid and gentle desalting of proteins and peptides. Utilizing a high-performance polyacrylamide gel-filtration matrix, the columns ensure exceptional sample recovery and full compatibility with downstream LC-MS/MS analysis and other biochemical assays.
Product Overview
During sample preparation for mass spectrometry, it is essential to efficiently remove salts, detergents, dyes, or unreacted small molecules that interfere with ionization efficiency, chromatographic performance, or detection sensitivity. Conventional desalting methods and certain resin materials often exhibit the following limitations:
1. Sample Loss and Non-Specific Adsorption
Many resin materials contain hydrophobic surfaces that promote non-specific binding of proteins or peptides, resulting in sample loss, a particularly critical issue for low-abundance or limited-volume samples.
2. Media Instability and Sample Contamination
Certain polysaccharide-based resins can undergo enzymatic degradation or support microbial growth, leading to contamination of the purified sample during storage or operation.
3. Limited Chemical Compatibility
Poor tolerance to extreme pH, organic solvents, or oxidizing agents restricts their use in specialized sample processing workflows and may introduce undesirable side reactions such as carbohydrate contamination.
4. Labor-Intensive Operation with Variable Performance
Non-prepacked media require manual column packing and equilibration, increasing workflow complexity and causing variability in desalting rate, capacity, and separation performance.
Advantages
✅ Ready-to-Use Format for High-Efficiency Operation
MtoZ Biolabs’ Polyacrylamide Spin Desalting Columns are prepacked with high-performance porous polyacrylamide beads, allowing direct use without additional column preparation. Desalting or buffer exchange is completed within minutes through simple centrifugation steps, improving standardization and reproducibility across samples.
✅ Hydrophilic Resin with Ultra-Low Adsorption
The highly hydrophilic matrix minimizes non-specific interactions with biomacromolecules including proteins, peptides, and nucleic acids, ensuring excellent recovery even for precious trace samples.
✅ Outstanding Chemical and Physical Stability
The polyacrylamide matrix remains stable in aqueous salt solutions, organic solvents, and across a broad pH range (pH 2-10). The resin is not enzymatically degradable and does not support microbial growth, effectively preventing sample contamination.
✅ Thermal Stability and Sterilization Compatibility
The resin tolerates autoclaving at 120 °C (pH 5.5-6.5), supporting sterile handling applications when required.
✅ Precise Molecular Exclusion for Efficient Purification
With controlled pore-size distribution, the resin retains large biomolecules such as proteins and antibodies while allowing salts, dyes, phenolic compounds, and unreacted labeling reagents to rapidly elute, achieving clean separation and efficient desalting.
✅ Broad Downstream Application Compatibility
The resulting samples are free of interfering contaminants and fully compatible with workflows including LC-MS/MS proteomics, enzyme activity assays, fluorescence and radioactivity detection, and cell-based functional assays.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
