MtoZ Biolabs' Dye and Biotin Removal Spin Columns quickly remove free dyes and excess biotin from labeled proteins or peptides, improving signal clarity and downstream assay performance.
Product Overview
Post labeling cleanup is often the step that determines data quality because residual small molecules can skew quantitation and suppress downstream detection. Removing unreacted fluorescent dyes free biotin reducing agents and crosslinkers is challenging for several reasons:
1. Cumbersome Handling
Multi-step desalting or exchange workflows add transfers, waiting time, and operator variability.
2. Residual Adsorption and Sample Loss
Hydrophobic dyes and reactive crosslinkers can bind to plastics or media, lowering recovery and distorting labeling ratios.
3. Contamination Introduction
Extra tubes, filters, and reagents increase the chance of keratin, salts, or carryover contaminants entering the sample.
4. Time-Dependent Side Reactions
Residual reactive chemistries may continue modifying proteins during slow cleanup, increasing heterogeneity.
Advantages
✔️Simple Operation
A simple load spin and collect format streamlines post reaction cleanup and eliminates the time and handling burden of dialysis or precipitation.
✔️High Recovery
Minimal transfers and gentle processing help retain labeled proteins and peptides, supporting reliable work with limited input or low abundance targets.
✔️Multifunctional Cleanup
One column efficiently removes free fluorescent dyes excess biotin reducing agents and residual crosslinkers to improve quantitation accuracy and downstream performance.
✔️Broad Compatibility
MtoZ Biolabs' Dye and Biotin Removal Spin Columns fit common workflows after fluorescent labeling biotinylation or crosslinking and support clean readouts in SDS-PAGE imaging, Western blotting, and LC-MS analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
