The Plant Protein Extraction Kit (Chlorophyll Removal) from MtoZ Biolabs is formulated to efficiently extract high-purity total protein from plant tissues while removing chlorophyll and other pigment-related contaminants that interfere with downstream analyses.
Product Overview
Protein extraction from plant tissues is technically demanding due to their structural rigidity and the presence of multiple interfering substances. These challenges commonly affect protein recovery, purity, and compatibility with analytical workflows:
1. Rigid Cell Wall Architecture
Plant cell walls rich in cellulose, hemicellulose, and pectin are highly resistant to mechanical disruption. Insufficient breakdown results in incomplete protein release and reduced extraction yield.
2. Phenolic Compound Interference
Polyphenols present in leaves, fruits, and other plant tissues oxidize during homogenization and readily bind proteins, forming insoluble aggregates that compromise solubility and functional integrity.
3. Chlorophyll and Polysaccharide Contamination
Chlorophyll, lipophilic pigments, starch, and polysaccharides frequently co-extract with proteins. These contaminants elevate sample viscosity, obscure electrophoretic bands, and negatively impact mass spectrometry performance.
4. Rapid Post-Lysis Protein Degradation
Endogenous plant proteases become active upon tissue disruption and can degrade target proteins rapidly, reducing the reliability of structural and functional studies.
Advantages
The MtoZ Biolabs Plant Protein Extraction Kit (Chlorophyll Removal) provides:
✅ Effective Disruption of Cell Wall Structures
Optimized buffer components facilitate thorough breakdown of cellulose-rich plant cell walls, enabling efficient release of intracellular proteins across diverse tissue types.
✅ Selective Chlorophyll Removal
A specialized pigment-removal system eliminates chlorophyll and related pigments, producing clear, low-background extracts ideal for electrophoresis, immunoassays, and LC-MS/MS.
✅ Reduction of Phenolic and Polysaccharide Interference
Polyphenol-neutralizing agents and carbohydrate-reducing components minimize crosslinking, viscosity issues, and co-extraction artifacts, improving overall sample quality.
✅ Protection Against Protein Degradation
A plant-specific protease inhibitor blend safeguards proteins during extraction, preserving structural integrity for reliable downstream analysis.
✅ Enhanced Compatibility with Multiple Workflows
The resulting protein extracts exhibit high purity and low viscosity, supporting SDS-PAGE, Western blotting, quantitative assays, and high-resolution proteomics.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
