The Plant Histone Extraction Kit from MtoZ Biolabs is specifically engineered to efficiently enrich high-purity histones from plant tissues. This kit streamlines conventionally labor-intensive workflows and effectively preserves the native landscape of histone post-translational modifications.
Product Overview
Histones and their post-translational modifications are central to plant epigenetic regulation, contributing to gene expression control, chromatin structural dynamics, and responses to environmental stresses. However, isolating high-quality histones from plant tissues remains technically challenging due to:
1. Difficult Cell Disruption
Plant tissues possess rigid cell walls that require efficient mechanical and chemical disruption to release intact nuclei; these complex initial steps can readily lead to histone loss.
2. Labor-Intensive and Lengthy Procedures
Conventional histone-extraction workflows typically involve multiple sequential steps, including nuclear isolation and acid extraction, resulting in time-consuming processes with limited reproducibility.
3. Co-Extraction of Abundant Contaminants
High-abundance non-histone proteins in plant tissues are readily extracted simultaneously, severely compromising sample purity and reducing sensitivity and accuracy in downstream modification-profiling analyses.
4. Potential Loss of Modification Information
Histone PTMs (e.g., methylation and acetylation) are highly labile; harsh or prolonged extraction conditions may cause modification alteration or loss, preventing accurate reflection of in vivo epigenetic states.
Advantages
✅ Targeted Lysis and Nuclear Enrichment
The Plant Histone Extraction Kit from MtoZ Biolabs employs an optimized strategy that efficiently disrupts plant cells and enriches nuclei, establishing a robust starting point for subsequent high-purity histone isolation.
✅ Integrated and User-Friendly Workflow
Nuclear separation and histone purification are seamlessly integrated into a streamlined protocol, significantly reducing operational time and labor while ensuring excellent reproducibility.
✅ High Specificity and Sample Purity
A proprietary purification technology effectively eliminates abundant contaminating proteins (e.g., Rubisco) and nucleic acids, delivering histone preparations that meet stringent requirements for high-resolution mass spectrometry.
✅ Preservation of the Native Modification Profile
Gentle extraction conditions and dedicated protective reagents ensure maximal retention of endogenous histone post-translational modifications, enabling reliable characterization of authentic epigenetic information.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
