The Plant Phosphoprotein Extraction Kit from MtoZ Biolabs is designed for efficient isolation of phosphorylated proteins from plant tissues, enabling high-quality extraction for phosphoproteomics, signaling pathway analysis, and functional studies of plant response mechanisms.
Product Overview
Phosphoproteins are essential for regulating cellular processes, particularly in response to environmental stimuli. However, isolating these proteins from plant tissues is challenging due to the following factors:
1. Low Abundance of Phosphoproteins
Phosphorylation is a dynamic modification, and phosphoproteins often exist at low levels in complex plant samples, making selective extraction essential to enrich phosphoproteins and avoid interference from non-phosphorylated proteins.
2. Presence of Interfering Compounds
Plant tissues contain high levels of polysaccharides, polyphenols, and pigments, which can co-extract with proteins and reduce the purity of the extracted phosphoproteins, interfering with downstream analyses like mass spectrometry.
3. Phosphatase Activity
Endogenous phosphatases in plant cells can dephosphorylate proteins during extraction, leading to the loss of valuable phosphorylation information.
Advantages
The MtoZ Biolabs Plant Phosphoprotein Extraction Kit provides:
✅ Efficient Enrichment of Phosphorylated Proteins
Optimized extraction conditions ensure high recovery of phosphorylated proteins with minimal contamination from non-phosphorylated proteins.
✅ Protection Against Protein Degradation
A blend of protease inhibitors preserves protein integrity and phosphorylation during the extraction process.
✅ Reduced Contamination from Plant Interfering Compounds
The extraction chemistry effectively removes polysaccharides, phenolic compounds, and other contaminants, resulting in pure phosphoprotein samples.
✅ Ideal for Phosphoproteomics and Functional Assays
Extracted phosphoproteins are compatible with mass spectrometry, Western blotting, kinase assays, and other functional studies, enabling in-depth analysis of plant signaling pathways.
✅ Versatile Across Various Plant Tissues
Suitable for a range of plant tissues, including leaves, stems, roots, and seeds, supporting diverse applications in plant stress response, signaling, and development studies.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
