The Plant Active Protein Extraction Kit from MtoZ Biolabs is designed to extract functionally active proteins from plant tissues under mild conditions, preserving native conformation and enzymatic activity for downstream functional analysis.
Product Overview
Isolating active proteins from plant tissues is technically challenging because protein activity is highly sensitive to extraction conditions. Common challenges include:
1. Loss of Protein Activity During Extraction
Harsh lysis conditions, extreme pH, or strong detergents can disrupt protein conformation, leading to loss of enzymatic or binding activity.
2. Interference from Plant-derived Compounds
Polyphenols, polysaccharides, and secondary metabolites present in plant tissues can interact with proteins, affecting activity measurements and assay reliability.
3. Protease and Phosphatase Activity
Endogenous enzymes released during tissue disruption can rapidly degrade or modify target proteins, altering their functional state.
4. Heterogeneity of Plant Tissues
Different plant organs and species vary widely in composition, making it difficult to maintain consistent extraction conditions that preserve protein activity across samples.
Advantages
The MtoZ Biolabs Plant Active Protein Extraction Kit provides the following benefits:
✅ Preservation of Native Protein Activity
Mild extraction conditions are optimized to maintain native protein conformation and functional activity.
✅ Reduced Interference from Plant Matrix Components
The formulation minimizes the impact of polyphenols, polysaccharides, and other interfering substances on active proteins.
✅ Protection Against Enzymatic Degradation
Integrated inhibitor systems limit unwanted protease and phosphatase activity during extraction.
✅ Consistent Performance Across Plant Samples
The kit supports reproducible extraction of active proteins from diverse plant tissues and species.
✅ Compatibility with Functional Assays
Extracted proteins are suitable for enzymatic assays, interaction studies, and activity-based analyses.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
