Target Information
| Target information | Mechanistic target of rapamycin (mTOR), also known as FRAP1, is a large serine/threonine protein kinase that integrates nutrient availability, energy status, and growth factor signaling. As the catalytic core of mTORC1 and mTORC2, mTOR coordinates cellular growth, protein synthesis, autophagy, metabolism, and survival-related signaling. It is one of the most widely studied kinases in signal transduction, metabolic regulation, and cell growth research. When mTOR-containing protein material requires enrichment before LC-MS/MS analysis, MtoZ Biolabs offers IP-MS Validated mTOR Antibody for immunoprecipitation-based workflows. This mTOR antibody validated by IP-MS supports users seeking an mTOR IP antibody, an mTOR antibody for immunoprecipitation, or an mTOR antibody in IP-MS workflows. It can support target capture, mTOR-associated protein analysis, and mass spectrometry-based characterization in mTOR signaling and kinase pathway studies. |
|---|---|
| Target name | FK506 binding protein 12-rapamycin associated protein 1 |
| Aliases | FRAP1, FRAP2, FRAP, FKBP12-rapamycin complex-associated protein, EC:2.7.11.1, SKS, RAFT1, RAPT1, mTOR |
| UniProt accession | P42345 |
Product Introduction
| Primary applications |
Before delivery, each antibody undergoes IP-MS validation to verify its ability to recognize and enrich the intended target protein |
|---|---|
| Other applications | WB, IHC, IF, ICC, ELISA, IF, PLA |
| Species reactivity | Human, Mouse, Rat, Pig, Monkey, Chicken, Bovine, Hamster, Goat, Rabbit |
| Clonality | Monoclonal, Polyclonal |
| Host | Rabbit, Mouse |
| Recommended dilution ratio | IP: 0.5μg-4μg antibody for 200μg-3000μg extracts of whole cells |
| Specification | 50/100/150/200 μL |
| Storage condition | Short-term storage at 2-8 °C. Long-term storage at -20 °C. Avoid repeated freeze-thaw cycles |
MtoZ Biolabs IP-MS Workflow Products
MtoZ Biolabs offers a fully integrated IP-MS workflow solution, combining antibodies validated for IP-MS with expertly paired IP kits and seamless downstream mass spectrometry services. Our platform empowers researchers to achieve reliable target enrichment, protein interaction mapping, and comprehensive proteomic profiling with confidence and efficiency.
IP-MS Validated Antibodies
- Antibodies rigorously tested for IP-MS applications
- Minimize experimental uncertainty and accelerate research timelines
- Broad applicability across multiple species and protein targets
Expertly Paired IP Kits
- Designed and validated to complement MtoZ IP-MS antibodies
- Detailed step-by-step experimental protocols for reproducible target enrichment
- Optimized workflows to maximize antibody performance
Comprehensive IP-MS Analysis Services
- Submit immunoprecipitated samples directly to MtoZ Biolabs
- High-resolution mass spectrometry for target verification, interaction mapping, and proteomics studies
- Structured, publication-ready reports for seamless documentation and data reproducibility
Antibody Validation Levels
To support different research goals, budgets, and evidence requirements, our antibodies are available with multiple validation levels. Each antibody undergoes IP-MS validation before delivery to confirm target-specific recognition and enrichment. For projects requiring additional confirmation, Western blot validation can be added to provide orthogonal evidence of target detection.
| Validation level | Included validation | Best for |
|---|---|---|
| IP-MS Only | Confirms target-specific enrichment by immunoprecipitation followed by mass spectrometry analysis. | IP-based protein enrichment and target specificity assessment. |
| Western Blot Only | Confirms target detection by Western blot under defined experimental conditions. | Routine target detection and expression-level assessment. |
| IP-MS + Western Blot | Combines mass spectrometry-based target enrichment evidence with Western blot-based detection evidence. | Customers requiring more comprehensive validation support. |
Antibody Validation Workflow
Validation Report Included
IP-MS
- IP experiment record
- Antibody usage information
- Original experimental image
- Mass spectrometry parameters
- Identified target peptides
- Representative MS/MS spectra
Western Blot
- WB experimental conditions
- Original blot image
- Qualitative target band recognition result
MtoZ Biolabs IP-MS Antibody Solutions
Traditional Antibody Supplier
Conventional workflowCustomers need to purchase small amounts of antibody for in-house testing
01Validation depends on customer-provided samples and workflows
02Suppliers usually stop support after product delivery
03Customers must find a separate provider for MS or interaction analysis
04Higher risk of repeated trial-and-error
05
MtoZ Biolabs IP-MS Antibody Solutions
RecommendedMtoZ Biolabs can provide IP-MS validation before or at the time of purchase
01Validation is supported by MS-based evidence and a detailed report
02Antibody purchase can be directly connected to downstream IP-MS services
03Antibody and downstream IP-MS analysis can be completed within a single workflow
04Reduces trial attempts and accelerates project progress
05
Why Choose MtoZ Biolabs?
Antibodies Validated
Target Proteins
Research Institutions Served
Full Validation Reports Included
Structured IP/Co-IP/IP-MS validation reports are included with every antibody for easy lab recordkeeping and project documentation.
Ultra-High Resolution MS Platform
IP-MS validation on high-resolution LC-MS/MS instrumentation for confident target enrichment and specificity assessment.
