Protein Array Analysis Service
- Protein Identification: Detect and identify both known and novel proteins from complex biological samples.
- Protein Quantification: Accurately measure protein levels to examine expression profiles and regulatory changes.
- PTM Analysis: Investigate post-translational modifications, revealing crucial changes in protein function and activity.
- Protein Interaction Analysis: Study protein-protein interactions, providing insights into molecular signaling networks and disease mechanisms.
- Performs high-throughput analysis of hundreds to thousands of proteins simultaneously.
- Easy to use with accurate protein quantification in complex biological samples.
- Requires minimal sample volume and simple sample preparation.
- Customizable to meet specific research needs.
Protein array analysis is a high-throughput technique that allows for the simultaneous examination of protein expression, interactions, and functions on a large scale. By immobilizing proteins or protein-specific ligands on a solid surface, such as a glass or plastic slide, protein array analysis provides valuable insights into protein-protein interactions, post-translational modifications (PTMs), and overall protein behavior in various biological contexts.
Protein arrays vary in design, from dot blots that display dozens of proteins to more advanced membrane protein arrays with hundreds of proteins. The most sophisticated protein microarrays can assess thousands of proteins simultaneously, offering an unprecedented level of throughput. This makes protein array analysis indispensable for proteomics research, biomarker discovery, and drug development.
MtoZ Biolabs offers an advanced Protein Array Analysis Service that employs microarray, spotted array, and other state-of-the-art technologies. Our service provides high-throughput, reproducible results by leveraging sophisticated detection methods and expert analysis. This enables the identification and quantification of proteins with remarkable sensitivity, making it an essential tool for both basic and applied scientific research. Our Protein Array Analysis Service can also be seamlessly integrated with mass spectrometry (MS) techniques, enhancing protein identification and providing in-depth analysis of protein interactions, post-translational modifications (PTMs), and protein expression levels. This powerful combination allows for more comprehensive data, offering a deeper understanding of complex biological systems. Our Protein Array Analysis Service offers several critical applications:
Figure 1. Schematic Representation of Protein Microarray
Service Advantages
Protein Array Analysis Service offers the following key advantages:
Case Study
Enhancing COVID-19 Diagnosis with Protein Array Analysis Technology
The coronavirus antigen microarray was validated to detect antibody profiles from both pre-pandemic and COVID-19 convalescent samples. By incorporating antigens from SARS-CoV-2, SARS-CoV, MERS-CoV, and common respiratory viruses, the array achieved near complete discrimination between these groups. This protein array analysis outperformed traditional PCR testing, offering a more accurate estimation of COVID-19 disease burden. The array can be used for diagnostics, epidemiological studies, and correlating antibody responses with clinical outcomes, advancing both research and public health efforts.
Figure 2. Heatmap for Coronavirus Antigen Microarray
FAQ
Q: What methods are used to image protein arrays?
Protein arrays are imaged using fluorescence or chemiluminescence detection. Fluorescence imaging involves scanning with a laser to detect emitted light from labeled proteins, while chemiluminescence captures light from enzyme reactions. These methods provide high sensitivity, enabling accurate quantification of protein expression, interactions, and modifications in high-throughput analysis.
Deliverables
1. Comprehensive Experimental Details
2. Materials, Instruments, and Methods
3. Total Ion Chromatogram & Quality Control Assessment (project-dependent)
4. Data Analysis, Preprocessing, and Estimation (project-dependent)
5. Bioinformatics Analysis
6. Raw Data Files
MtoZ Biolabs, an integrated chromatography and mass spectrometry (MS) services provider.
Related Services
Protein Microarray Analysis Service
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