Target Information
| Target information | Mouse Double Minute 2 homolog (MDM2), also known as HDM2 in human studies, is an E3 ubiquitin ligase and key regulator commonly associated with p53 pathway control. Phosphorylation at serine 166, referred to as phospho S166, is a site frequently examined in studies of MDM2 regulation, signaling-dependent activity, and changes in protein behavior under different cellular conditions. Antibodies directed to MDM2 phosphorylated at S166 are commonly used to distinguish this modified form from total MDM2 in signaling and phosphoprotein research. This IP-MS validated MDM2 (phospho S166) antibody from MtoZ Biolabs is designed for researchers seeking a phospho-specific MDM2 antibody for immunoprecipitation and downstream mass spectrometry analysis. As an MDM2 phospho S166 antibody validated by IP-MS, it supports selective enrichment of the modified target in IP-MS workflows and can be applied in studies of phosphorylation-dependent regulation, target capture, and comparative phosphoproteomics. For scientists searching for an MDM2 (phospho S166) IP antibody or an MDM2 antibody in IP-MS workflows, this product provides a practical option for connecting phospho-specific immunoprecipitation with protein identification and modification-focused analysis. |
|---|---|
| Target name | MDM2 proto-oncogene |
| Aliases | E3 ubiquitin-protein ligase Mdm2, Double minute 2 protein, Hdm2, Oncoprotein Mdm2, RING-type E3 ubiquitin transferase Mdm2, p53-binding protein Mdm2 |
| UniProt accession | Q00987 |
Product Introduction
| Primary applications |
Before delivery, each antibody undergoes IP-MS validation to verify its ability to recognize and enrich the intended target protein |
|---|---|
| Other applications | IHC, ChIP |
| Species reactivity | Human |
| Clonality | Monoclonal |
| Host | Mouse |
| Recommended dilution ratio | 1:100 |
| Specification | 5/20/50/100 μL |
| Storage condition | Short-term storage at 2-8 °C. Long-term storage at -20 °C. Avoid repeated freeze-thaw cycles |
MtoZ Biolabs IP-MS Workflow Products
MtoZ Biolabs offers a fully integrated IP-MS workflow solution, combining antibodies validated for IP-MS with expertly paired IP kits and seamless downstream mass spectrometry services. Our platform empowers researchers to achieve reliable target enrichment, protein interaction mapping, and comprehensive proteomic profiling with confidence and efficiency.
IP-MS Validated Antibodies
- Antibodies rigorously tested for IP-MS applications
- Minimize experimental uncertainty and accelerate research timelines
- Broad applicability across multiple species and protein targets
Expertly Paired IP Kits
- Designed and validated to complement MtoZ IP-MS antibodies
- Detailed step-by-step experimental protocols for reproducible target enrichment
- Optimized workflows to maximize antibody performance
Comprehensive IP-MS Analysis Services
- Submit immunoprecipitated samples directly to MtoZ Biolabs
- High-resolution mass spectrometry for target verification, interaction mapping, and proteomics studies
- Structured, publication-ready reports for seamless documentation and data reproducibility
Antibody Validation Levels
To support different research goals, budgets, and evidence requirements, our antibodies are available with multiple validation levels. Each antibody undergoes IP-MS validation before delivery to confirm target-specific recognition and enrichment. For projects requiring additional confirmation, Western blot validation can be added to provide orthogonal evidence of target detection.
| Validation level | Included validation | Best for |
|---|---|---|
| IP-MS Only | Confirms target-specific enrichment by immunoprecipitation followed by mass spectrometry analysis. | IP-based protein enrichment and target specificity assessment. |
| Western Blot Only | Confirms target detection by Western blot under defined experimental conditions. | Routine target detection and expression-level assessment. |
| IP-MS + Western Blot | Combines mass spectrometry-based target enrichment evidence with Western blot-based detection evidence. | Customers requiring more comprehensive validation support. |
Antibody Validation Workflow
Validation Report Included
IP-MS
- IP experiment record
- Antibody usage information
- Original experimental image
- Mass spectrometry parameters
- Identified target peptides
- Representative MS/MS spectra
Western Blot
- WB experimental conditions
- Original blot image
- Qualitative target band recognition result
MtoZ Biolabs IP-MS Antibody Solutions
Traditional Antibody Supplier
Conventional workflowCustomers need to purchase small amounts of antibody for in-house testing
01Validation depends on customer-provided samples and workflows
02Suppliers usually stop support after product delivery
03Customers must find a separate provider for MS or interaction analysis
04Higher risk of repeated trial-and-error
05
MtoZ Biolabs IP-MS Antibody Solutions
RecommendedMtoZ Biolabs can provide IP-MS validation before or at the time of purchase
01Validation is supported by MS-based evidence and a detailed report
02Antibody purchase can be directly connected to downstream IP-MS services
03Antibody and downstream IP-MS analysis can be completed within a single workflow
04Reduces trial attempts and accelerates project progress
05
Why Choose MtoZ Biolabs?
Antibodies Validated
Target Proteins
Research Institutions Served
Full Validation Reports Included
Structured IP/Co-IP/IP-MS validation reports are included with every antibody for easy lab recordkeeping and project documentation.
Ultra-High Resolution MS Platform
IP-MS validation on high-resolution LC-MS/MS instrumentation for confident target enrichment and specificity assessment.
Explore More IP-MS Validated Antibodies
Complementary Protein Interaction Analysis Services
In addition to IP-MS validated antibodies, MtoZ Biolabs offers supporting interaction analysis services for downstream protein enrichment and protein interaction studies.
