Yeast 2 Hybrid Analysis Service

Yeast 2 Hybrid Analysis (Y2H) is a widely used molecular biology technique designed to detect and study protein-protein interactions (PPIs) in a cellular context. Yeast 2 hybrid analysis method leverages the modular structure of transcription factors, which are composed of two separate domains: the DNA-binding domain (DBD), responsible for binding to specific DNA sequences, and the activation domain (AD), which recruits the transcriptional machinery to initiate gene expression. In Y2H, these domains are functionally split and fused to two proteins of interest—referred to as the "bait" (fused to the DBD) and the "prey" (fused to the AD). When the bait and prey proteins physically interact within the yeast nucleus, the DBD and AD are brought into proximity, reconstituting a functional transcription factor. This reconstitution activates the expression of a reporter gene, such as GFP or lacZ, which can be detected through selective growth media or colorimetric assays. The strength and specificity of the interaction can also be quantitatively assessed by measuring reporter gene activity. Yeast 2 hybrid analysis enables systematic discovery and validation of protein interactions, by screening prey libraries against a single bait protein or performing reciprocal experiments.

 



 

Dhakal, S. et al. Microorganisms. 2022.

 

Figure 1. The Classical Yeast 2 Hybrid Analysis System for Biosensing Protein Interactions in Vivo

 

Yeast 2 hybrid analysis  method is instrumental in identifying novel protein interactors, validating previously reported PPIs, and constructing intricate protein interaction networks. Yeast 2 hybrid analysis is particularly valuable for studying signaling pathways, discovering disease-relevant biomarkers, and mapping protein complexes. Its high-throughput nature and ability to directly detect interactions make it a powerful tool for functional genomics and therapeutic target discovery.

 

Service at MtoZ Biolabs

MtoZ Biolabs, an integrated Chromatography and Mass Spectrometry (MS) Services Provider, provides advanced proteomics, metabolomics, and biopharmaceutical analysis services to researchers in biochemistry, biotechnology, and biopharmaceutical fields. MtoZ Biolabs provides Yeast 2 Hybrid Analysis Service that tailored to meet the diverse needs of researchers. Our Yeast 2 Hybrid Analysis Service offers access to comprehensive yeast libraries, high-efficiency screening platforms, and expert data interpretation to ensure reliable and reproducible results. Whether you aim to explore novel interactions, validate specific hypotheses, or build large-scale interaction maps, our service provides the expertise and resources necessary to advance your research. MtoZ Biolabs is your trusted partner in accelerating scientific discovery.

 

Service Advantages

1. Comprehensive Library Construction

MtoZ Biolabs provides high-quality library construction, starting from RNA extraction to cDNA synthesis and plasmid library creation. Our optimized workflows ensure comprehensive and diverse coverage, enabling the identification of a wide range of protein-protein interactions (PPIs).

 

2. In Vivo Interaction Validation

The ability to validate protein-protein interactions in a cellular environment ensures accurate representation of actual biological processes, providing highly reliable and biologically meaningful results.

 

3. Sensitive Detection of Weak and Transient Interactions

By capturing the cumulative effects of gene expression products, Yeast 2 Hybrid Analysis Service is highly sensitive to weak or transient protein interactions that are often missed by other techniques, enabling a more comprehensive mapping of interaction networks.

 

4. Advanced Analysis Platform

MtoZ Biolabs established an advanced Yeast 2 Hybrid Analysis Service platform, guaranteeing reliable, fast, and highly accurate analysis service.

 

5. One-Time-Charge

Our pricing is transparent, no hidden fees or additional costs.

 

6. High-Data-Quality

Deep data coverage with strict data quality control. AI-powered bioinformatics platform integrates all yeast 2 hybrid analysis data, providing clients with a comprehensive data report.

 

Applications

1. Identify and confirm interactions between specific pairs of known proteins.

2. Explore protein-protein interaction pathways using cDNA libraries with known protein genes.

3. Investigate interaction domains through mutational analysis of target proteins.

4. Discover functions of novel genes by screening interaction partners with known functional genes.

 

FAQ

Q: What methods are used to validate interaction results identified through yeast 2 hybrid analysis?

Post-screening, interaction results identified through yeast 2 hybrid analysis are validated by reversing bait and prey roles to confirm interaction specificity. Additional negative controls and re-screening steps are employed to eliminate false positives. For higher confidence, complementary methods such as co-immunoprecipitation (Co-IP) or pull-down assays can be used. These orthogonal techniques provide further validation of the identified interactions in different biological contexts, ensuring reliability and robustness of the results.

 

Our Yeast 2 Hybrid Analysis Service is designed to provide more rapid, high-throughput, and cost-effective analysis, with exceptional data quality and minimal sample consumption. Free project evaluation, welcome to learn more details.