The E.coli HCP (Alkaloid Cleavage) ELISA Kit from MtoZ Biolabs is a quantitative immunoassay developed for measuring residual Escherichia coli host cell proteins in samples processed by alkaloid or alkaline cleavage workflows, supporting impurity monitoring in plasmid and recombinant product manufacturing.
Product Overview
Residual E. coli host cell proteins are key process-related impurities in microbial expression systems, particularly in workflows involving alkaloid or alkaline cleavage. Accurate HCP measurement requires an assay compatible with both the treated protein profile and the associated sample matrix. Key considerations include:
1. Process-Modified HCP Recognition
Alkaloid or alkaline cleavage can alter HCP structure and immunoreactivity, requiring assays that maintain reliable antibody binding after treatment.
2. Compatibility With Alkaline Lysis-Derived Samples
Crude and intermediate fractions generated during plasmid extraction present complex matrices that can affect assay specificity and signal stability.
3. Coverage Across Common E. coli Clones
Broad strain reactivity is essential for consistent HCP quantitation across commonly used laboratory and production strains.
Advantages
The MtoZ Biolabs E.coli HCP (Alkaloid Cleavage) ELISA Kit provides the following advantages:
1. Proces-Relevant Design
Optimized for samples subjected to alkaloid or alkaline cleavage, improving alignment between assay performance and actual process conditions.
2. Broad Strain Applicability
Suitable for HCP quantitation across widely used E. coli clone backgrounds, supporting cross-project comparability.
3. Defined Quantitative Performance
Provides reliable measurement across a working concentration range appropriate for crude and intermediate process samples.
4. Standardized ELISA Workflow
Plate-based format enables consistent execution, moderate throughput, and straightforward integration into routine testing.
5. Storage and Stability for Routine Use
Reagent stability under refrigerated conditions supports repeated use, method transfer, and inventory management in regulated environments.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
