The CHO HCP ELISA Kit (One-Step ELISA) from MtoZ Biolabs is a double-antibody sandwich ELISA kit designed to detect and quantify residual Chinese hamster ovary (CHO) host cell proteins in biotherapeutic samples for process monitoring and product purity assessment.
Product Overview
Host cell proteins are key process-related impurities in recombinant biologics produced in CHO cells, and accurate CHO HCP quantitation is essential for verifying product purity and meeting regulatory expectations. However, CHO HCP testing can be complicated. Common challenges include:
1. Complex HCP Mixtures
CHO cells shed a wide range of endogenous proteins with diverse properties, making broad and consistent detection challenging across processes and lots.
2. Trace Level Residuals
HCPs often remain at very low levels in purified drug substance, requiring sensitive measurement to avoid false negatives and missed excursions.
3. Matrix Effects
Buffers, salts, detergents, and high product concentrations can distort readouts by increasing background or suppressing antibody binding.
4. Workflow Burden
Multi-step ELISA formats with repeated incubations and washes can slow turnaround and introduce day-to-day variability.
Advantages
✔️One Step Workflow
MtoZ Biolabs' CHO HCP ELISA Kit (One-Step ELISA) reduces unnecessary incubations and wash cycles, supporting faster turnaround and more consistent routine testing.
✔️Sensitive Quantitation
Delivers reliable detection for low level residual HCPs to strengthen clearance verification and lot release confidence.
✔️Low Background
Optimized reagents and assay conditions help reduce nonspecific binding and background signal, improving accuracy in real sample matrices.
✔️Matrix Tolerant Performance
Maintains robust performance in common formulation and process buffers, supporting consistent results across purification steps and final product conditions.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
