MtoZ Biolabs' CHO-K1 HCP ELISA Kit (One-Step ELISA) is a ready-to-use double-antibody sandwich ELISA for fast, quantitative monitoring of residual CHO-K1 host cell proteins (HCP) across downstream purification, formulation, and release testing.
Product Overview
Host cell proteins are critical process-related impurities in biologics manufactured using the CHO-K1 cell line, a widely used Chinese hamster ovary subclone for recombinant protein expression, and reliable CHO-K1 HCP quantitation is important for confirming clearance, supporting product quality, and aligning with regulatory requirements. Common challenges of CHO-K1 HCP testing include:
1. Complex HCP Background
CHO-K1 produces a diverse HCP profile that can shift with process changes, making consistent quantitation across lots and runs difficult.
2. Trace-Level Targets
HCPs can persist at very low levels in purified drug substance, increasing the risk of false negatives when sensitivity is limited.
3. Matrix Effects
Buffers, salts, detergents, and high product concentration can elevate nonspecific signal or reduce antibody binding, impacting accuracy.
4. Workflow Burden
Multi-step incubations and repeated washes slow turnaround and introduce day-to-day variability between operators.
Advantages
✔️One-Step Speed
MtoZ Biolabs CHO-K1 HCP ELISA Kit (One-Step ELISA) reduces excessive incubations and wash cycles to support faster results with fewer handling points.
✔️Sensitive Quantitation
Optimized assay conditions help detect low-abundance HCPs in highly purified samples, improving confidence in clearance assessment.
✔️Low Nonspecific Signal
Blocking and assay chemistry are tuned to minimize background and matrix-driven artifacts for cleaner, more reliable readouts.
✔️Consistent Performance
Standardized kit components support run-to-run comparability and easier trending across process development and manufacturing campaigns.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
