The Pichia pastoris HCP ELISA Kit (One-Step ELISA) from MtoZ Biolabs is a double-antibody sandwich ELISA kit built to detect and quantify residual Pichia host cell proteins in biotherapeutic samples, helping teams verify purity and monitor process clearance from development through manufacturing.
Product Overview
Host cell proteins (HCPs) are a major class of impurities introduced during recombinant protein expression, and Pichia pastoris-based production can leave a complex, highly variable HCP background that must be tightly controlled. Reliable HCP quantitation is essential for demonstrating product purity, monitoring downstream clearance, and supporting regulatory expectations. In practice, measuring Pichia HCPs often faces several recurring challenges:
1. Time and Hands-On Burden
Multi-step incubation and wash cycles slow release testing and increase operator-dependent variation.
2. Complex HCP Profile
Pichia produces a broad range of endogenous proteins with wide abundance and physicochemical diversity, which can complicate consistent detection across processes and lots.
3. Low-Level Targets
HCPs are often present at trace levels in purified drug substance, requiring sensitive detection to avoid false negatives.
4. Matrix Interference
Buffers, salts, detergents, and high product concentrations can increase nonspecific signal or suppress antibody binding, reducing accuracy.
Advantages
✔️One-Step Workflow
MtoZ Biolabs' Pichia pastoris HCP ELISA Kit (One-Step ELISA) streamlines the assay with fewer wash cycles and incubation steps, helping shorten turnaround time while reducing day-to-day handling variability.
✔️Broad HCP Coverage
Built with anti-Pichia antibodies designed to recognize a wide spectrum of Pichia pastoris host cell proteins, supporting consistent monitoring across different processes, lots, and purification stages.
✔️High Sensitivity at Trace Levels
Sensitive one-step sandwich ELISA design helps quantify low-abundance HCPs in purified drug substance, reducing the risk of false negatives during release and stability testing.
✔️Matrix Tolerant Performance
Optimized blockers and wash conditions help reduce nonspecific binding and background signal while maintaining antibody-antigen interactions in the presence of salts, detergents, and high product concentrations, improving accuracy in real samples.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
