The Micro C-Trap LysN Digestion and Desalting Kit launched by MtoZ Biolabs is designed for micro-scale complex protein samples. It integrates LysN digestion and desalting workflows, reduces sample loss, and helps support low-input proteomics analysis.
Product Overview
Micro-scale protein samples typically contain low protein amounts and are more likely to carry residual salts and buffer components, resulting in a higher proportion of background constituents. These characteristics pose significant challenges to traditional LysN digestion and desalting workflows:
1. Micro-Scale Recovery Loss
After extraction, the overall amount of micro-scale samples is limited. In traditional stepwise workflows, repeated tube changes, transfers, and multiple opening steps more readily cause adsorption, residual retention, and sample loss, making low-abundance components more difficult to preserve.
2. Buffer Residue Interference
Salts, detergents, or elution buffer components account for a higher relative proportion in micro-scale systems and are more likely to interfere with subsequent LysN digestion and peptide binding, leading to unstable output.
3. Digestion Consistency Variability
Differences in sample sources lead to variation in protein concentration and matrix composition. Under traditional conditions, it is difficult to uniformly control the reaction environment, making missed cleavages and shifts in peptide distribution more likely and affecting comparability.
4. Signal Suppression
Residual salts and small-molecule interferences can cause ion suppression and elevate background signals, which under low-input conditions more readily result in signal fluctuations and reduced reproducibility.
Advantages
1. Integrated In-Tube Workflow
The MtoZ Biolabs' Micro C-Trap LysN Digestion and Desalting Kit enables LysN digestion and desalting to be completed within the same tube, reducing handling steps and minimizing sample loss.
2. Optimized LysN-Directed Cleavage
The reaction system is optimized based on the characteristic of LysN cleaving at the N-terminus of lysine residues, helping to achieve more consistent cleavage output and mitigate variability caused by missed cleavages.
3. Enhanced Desalting and Interference Removal
The built-in desalting step simultaneously attenuates the impact of salts and residual buffer components, reducing ion suppression and background noise and improving LC-MS/MS detection stability.
4. Improved Batch Comparability
Standardized reaction and washing workflows reduce operator-dependent variability, making the kit more suitable for batch comparisons and cross-batch stability requirements in micro-scale samples.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
