The C-Trap Chymotrypsin Digestion and Desalting Kit from MtoZ Biolabs offer efficient, high-throughput solutions for protein digestion using chymotrypsin and subsequent desalting, available in multiple formats (microplate, 96-well, and 384-well) to accommodate different sample volumes and throughput needs, providing reliable, reproducible results for mass spectrometry analysis.
Product Overview
Protein digestion and desalting are essential steps for preparing high-quality samples for mass spectrometry (MS) analysis. However, challenges often arise, especially when dealing with large-scale samples or low-abundance proteins:
1. Inconsistent Digestion Efficiency
Uneven protein digestion can result in incomplete cleavage, impacting peptide yield and MS analysis accuracy.
2. Sample Contamination
Impurities such as salts, detergents, or residual matrix components can interfere with mass spectrometry, increasing background noise and reducing sensitivity.
3. Labor-Intensive, Multi-Step Procedures
Conventional workflows involving several manual steps increase the potential for human error and sample loss, leading to inefficiencies and delays.
4. Loss of Low-Abundance Proteins
Sample handling and transfer steps increase the risk of losing valuable low-abundance peptides, impacting the detection of subtle biological variations.
Advantages
The MtoZ Biolabs C-Trap Chymotrypsin Digestion and Desalting Kit provides the following advantages:
✅ Flexible Formats for Varying Throughputs
Available in microplate, 96-well, and 384-well formats, the kit accommodates small to large sample volumes, ensuring flexibility in different experimental setups.
✅ Optimized for Chymotrypsin Digestion
The kit is specifically designed for chymotrypsin digestion, efficiently cleaving proteins at aromatic residues, resulting in high-quality peptides for MS analysis.
✅ Integrated Desalting for Clean Peptides
The built-in desalting step effectively removes salts, detergents, and contaminants, ensuring that eluted peptides are ready for high-sensitivity MS analysis.
✅ Streamlined, Integrated Workflow
The integrated digestion and desalting process eliminates the need for sample transfers or tube opening, reducing handling time and minimizing sample contamination or loss.
✅ Reproducible and Consistent Results
The kit ensures consistent digestion and desalting, providing reliable, reproducible results across different batches, operators, and experiments.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
