The Micro C-Trap Glu-C Digestion and Desalting Kit launched by MtoZ Biolabs is designed for low-input protein samples. It integrates Glu-C digestion and desalting, reduces sample loss, and helps improve the stability of mass spectrometric analysis.
Product Overview
Low-input complex protein samples typically contain smaller protein amounts and a higher relative proportion of matrix residues, which poses specific challenges to the stability of Glu-C digestion and desalting following protein extraction.
1. Amplified Low-Input Loss
Traditional stepwise workflows require multiple tube openings and transfers. Under low-input conditions, nonspecific adsorption residues and irreversible losses are more likely to occur, making low-abundance peptides more difficult to retain.
2. Fluctuating Digestion Efficiency
The impact of residual salts, detergents, and denaturants is amplified in low-volume systems, which can alter Glu-C cleavage kinetics and lead to missed cleavages and unstable peptide yields.
3. Recovery Bias of Acidic Peptides
Peptides generated by Glu-C tend to be acidic. Under conventional desalting and elution conditions, slight mismatches can result in insufficient binding or uneven elution, reducing recovery and reproducibility.
4. Background Interference During MS Analysis
Residual salts and small-molecule contaminants can cause ion suppression and elevate background signals, leading to spray instability and signal fluctuations in low-input samples.
Advantages
✅ Integrated In-Tube Workflow
The micro C-trap Glu-C digestion and desalting kit from MtoZ Biolabs enables consecutive digestion and desalting within the same tube, reducing handling steps and minimizing sample loss.
✅ Controlled Cleavage
The reaction system is optimized based on the cleavage characteristics of Glu-C, helping to achieve more consistent peptide outputs in low-input, complex backgrounds.
✅ Cleaner Desalting
Built-in desalting simultaneously reduces the impact of residual salts and buffers, lowering ion suppression and background noise and improving LC-MS/MS stability.
✅ Improved Reproducibility
Standardized reaction and washing paths reduce operator-dependent variability, making the kit well suited for reproducibility and comparability requirements in low-input samples.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
