The 384-Well C-Trap Glu-C Digestion and Desalting Kit from MtoZ Biolabs is a high-throughput solution for efficient protein digestion and desalting using Glu-C protease in a 384-well format, providing streamlined sample preparation for mass spectrometry analysis with high-quality, reproducible results.
Product Overview
Efficient digestion and desalting are crucial steps in preparing proteins for mass spectrometry (MS) analysis. However, as sample throughput increases, managing multiple steps and handling large sample volumes can present challenges:
1. Inconsistent Digestion Across Large Sample Sets
Variability in digestion efficiency can lead to incomplete or overdigested proteins, affecting peptide yield and quality, especially when processing a high number of samples.
2. Sample Contamination
Impurities such as salts, detergents, or residual matrix components can interfere with mass spectrometry, resulting in background noise and lower sensitivity.
3. Time-Consuming Sample Preparation
Traditional multi-step digestion and desalting protocols require extensive manual handling, increasing the time spent per sample and introducing potential for error.
4. Sample Loss in Low-Abundance Proteins
Handling and transferring samples through multiple steps increases the risk of losing valuable peptides, particularly when working with low-abundance proteins.
Advantages
The MtoZ Biolabs 384-Well C-Trap Glu-C Digestion and Desalting Kit offers the following advantages:
✅ High-Throughput Capability
The 384-well format allows simultaneous processing of a large number of samples, making it ideal for large-scale proteomic studies and high-throughput applications.
✅ Efficient Glu-C Digestion
Optimized for Glu-C protease, the kit ensures complete and efficient digestion of proteins at the C-terminus of glutamic acid and aspartic acid residues, producing high-quality peptides for mass spectrometry.
✅ Integrated Desalting
The integrated desalting step effectively removes salts, detergents, and other contaminants, improving the quality of mass spectrometry results and reducing background interference.
✅ Simplified Workflow
The closed-system design eliminates the need for multiple sample transfers or tube openings, reducing handling time and minimizing the risk of sample loss or contamination.
✅ Consistent and Reproducible Results
With optimized reagents and a standardized format, the kit ensures reliable and reproducible results across experiments, providing consistent peptide yields and high-quality data.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
