MtoZ Biolabs has launched the Micro C-Trap Lys-C Digestion and Desalting Kit which is specifically designed for low-input samples. This kit integrates Lys-C digestion and desalting into a single workflow, reducing sample loss and improving recovery, and is suitable for mass spectrometry sample preparation.
Product Overview
Low-abundance protein samples are often characterized by small volumes, high susceptibility to adsorption, and relatively prominent background interference. These features place higher demands on integrated Lys-C digestion and desalting workflows.
1. Severe Sample Loss
During handling, low-input samples are prone to irreversible loss caused by liquid retention and adsorption to tube surfaces. This issue is particularly pronounced for high-abundance proteins, leading to reduced recovery of low-intensity peptide signals.
2. Background Interference
Salts, detergents, and other contaminating components commonly present in complex samples can interfere with enzymatic digestion and desalting processes, adversely affecting downstream analytical results and increasing background noise.
3. Complex Multi-Step Operations
Conventional digestion and desalting workflows involve multiple transfer, pipetting, and incubation steps, increasing operational complexity and the likelihood of error, while further complicating the handling of low-input samples.
4. Poor Operational Consistency
Low-input samples require highly controlled handling conditions. Manual operations are prone to introducing variability between batches, compromising experimental reproducibility.
Advantages
1. Integrated Design
MtoZ Biolabs' micro C-trap Lys-C digestion and desalting kit integrates digestion and desalting in the same vessel, simplifying operation steps and significantly reducing sample loss.
2. Efficient Lys-C Digestion
Reaction conditions are optimized based on the properties of Lys-C protease to enable stable, directed cleavage at lysine sites, maintaining strong digestion efficiency and consistency even under low-input or denaturing conditions.
3. Broad Adaptability
This kit is particularly suitable for low-abundance proteins and low-input samples, providing high recovery and stable digestion performance, and is applicable to different types of complex samples.
4. Consistent and Stable Results
Standardized operating procedures and reaction conditions reduce human error and operational variation, ensuring consistency between batches and high experimental reproducibility.
5. Compatibility with Downstream Analysis
The resulting peptides can be directly used for downstream analyses such as LC-MS/MS, reducing additional handling steps and ensuring high-quality data with low background.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
