MtoZ Biolabs' C-Trap Lys-C Digestion and Desalting Kit is specially designed for protein samples with structural stability or under denaturing conditions, enabling Lys-C directed digestion and simultaneous desalting, improving the efficiency of trace sample preparation.
Product Overview
When processing complex protein samples, the samples are often associated with characteristics such as compact conformation, complex solution components, small volume, and uneven site exposure, which places higher demands on the Lys-C digestion and desalting integration process.
1. Insufficient Site Accessibility
Some proteins have tight folding or aggregation, and lysine-related cleavage sites are not sufficiently exposed, leading to partial under-digestion or uneven digestion, affecting the distribution and consistency of subsequent peptides.
2. Amplified Losses in Trace Samples
When the sample volume is low, any opening, transfer, or residual liquid can amplify the loss. Wall adsorption and residual liquid may directly reduce peptide recovery, and weak signals are more likely to be lost.
3. Interference from Denaturing Components
Common salts, detergents, or denaturants in the sample can affect enzyme activity and peptide binding behavior, increasing desalting difficulty and potentially raising the background in subsequent detection.
4. Process Variability Leading to Differences
For high-throughput samples, manual steps can lead to batch-to-batch differences, affecting the stability and reproducibility of the experimental results.
Advantages
1. Integrated Design
MtoZ Biolabs' C-trap Lys-C digestion and desalting kit completes both Lys-C digestion and desalting in the same container, reducing uncertainty introduced by intermediate steps.
2. Efficient Lys-C Digestion
The kit is optimized for Lys-C protease, ensuring specific cleavage at lysine sites, providing more controlled peptide starting points for subsequent digestion or mass spectrometry analysis.
3. Direct Interference Removal
The built-in desalting path helps mitigate the effects of salts and denaturing components, reducing background interference and making the product more suitable for downstream analysis.
4. Stable Batch-to-Batch Performance
Standardized reaction and elution conditions improve process control, ensuring more consistent digestion and recovery performance across different operators and batches.
5. Smooth Downstream Integration
The resulting peptides can be directly used in subsequent LC-MS/MS analysis workflows, minimizing time and error accumulation from additional processing steps.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
