MtoZ Biolabs' BHK Residual DNA Fragment Detection Kit (PCR-Fluorescent Probe Method) is a quantitative detection kit based on the fluorescent probe method. It can analyze BHK cell residual DNA and its fragment size distribution in samples with high sensitivity. This kit is widely used in the quality control and safety evaluation research of various biological products.
Product Overview
During the research, development, and production of biological products, BHK (Baby Hamster Kidney) cells are commonly used as host cells or expression systems. Effectively monitoring and removing their residual DNA is crucial for ensuring drug safety. However, this detection process faces several challenges:
1. DNA Fragmentation
Host genomic DNA often breaks into small fragments during processes like cell lysis or purification. Traditional detection methods may miss these small fragments, leading to underestimated values or inaccurate residual DNA levels.
2. Matrix Effects and PCR Inhibition
Samples like recombinant proteins, antibodies, and vaccines contain complex components (proteins, salts, buffers) that can inhibit PCR amplification, causing false negatives or inaccurate quantification.
3. Sensitivity for Trace Residues
After purification, residual DNA in the final product is extremely low (pg or fg levels). Detecting these small amounts requires highly sensitive methods with a good signal-to-noise ratio.
4. Fragment Distribution Risk Assessment
Regulatory agencies are focusing not only on the total amount of DNA but also its fragment size distribution. Longer fragments (>200 bp) carry a higher risk, making the detection of various fragment sizes challenging.
5. Interference from Species Similarity
BHK and CHO cells have similar genomic sequences, which can cause cross-reactivity if the detection targets are not specific enough, reducing assay accuracy.
Advantages
✅ Comprehensive Fragment Size Analysis
MtoZ Biolabs' BHK Residual DNA Fragment Detection Kit quantifies four fragment sizes (81bp, 134bp, 216bp and 589bp) in parallel.
✅ High Sensitivity
Detection sensitivity down to the fg level across a broad dynamic range.
✅ Integrated Internal Process Control (IPC)
Enables real-time monitoring of amplification performance and sample inhibition, ensuring data reliability.
✅ High Analytical Specificity
BHK-specific primers and fluorescent probes provide excellent specificity with minimal cross-reactivity.
✅ Seamless Integration
Compatible with mainstream real-time PCR platforms and designed for use with the Host Cell Residual DNA Sample Preprocessing Kit, streamlining workflow integration.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
