The 96-Well C-Trap Lys-C Digestion and Desalting Kit launched by MtoZ Biolabs is designed for medium- to high-throughput complex protein samples, enabling integrated Lys-C digestion and desalting to improve the efficiency and consistency of mass spectrometry sample preparation.
Product Overview
Medium- to high-throughput protein samples originate from diverse sources, exhibit wide variations in protein abundance, and often show inconsistent backgrounds of salts, detergents, and denaturants, frequently accompanied by co-extracted impurities. These characteristics pose significant challenges for Lys-C digestion and desalting:
1. Insufficient Digestion Stability
Under traditional conditions, it is difficult to uniformly control different salt concentrations, urea levels, and detergent backgrounds, which readily leads to fluctuations in digestion rate and missed cleavages, resulting in inconsistent peptide yields.
2. High Risk of Sample Loss
Repeated opening, transfer, and container changes introduce risks of adsorption, residual loss, and contamination, making micro-scale samples and low-abundance peptides more prone to irreversible loss.
3. Uneven Desalting Performance
Insufficient cleanup leaves residual salts and denaturants that elevate background signals and cause ion suppression, whereas excessive cleanup can reduce peptide recovery, thereby limiting data stability.
4. Coverage Bias
Proteins with stable conformations or a tendency to aggregate are more likely to exhibit limited site accessibility in traditional workflows, leading to localized missed cleavages and shifts in peptide distribution.
Advantages
1. Integrated In-Plate Workflow
Lys-C digestion and desalting are completed sequentially within the same 96-well plate, reducing stepwise transitions and intermediate transfers, and lowering the risk of contamination and human error.
2. Efficient Lys-C Digestion
The workflow is optimized based on the characteristics of Lys-C retaining activity under denaturing conditions and having well-defined cleavage sites, enabling more stable and directional cleavage at lysine residues and facilitating more controllable peptide starting points.
3. Improved Inter-Well Consistency
Standardized reaction and washing workflows reduce inter-well variability, making them more suitable for the comparability requirements of batch sample analysis.
4. More Direct Impurity Removal
MtoZ Biolabs' 96-well C-trap Lys-C digestion and desalting kit has a built-in desalting step,which can simultaneously reduce the impact of salts and interfering components, facilitating downstream LC-MS/MS analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
