The 384-Well C-Trap LysN Digestion and Desalting Kit launched by MtoZ Biolabs is designed for ultra-high-throughput protein samples. It enables parallel LysN digestion and desalting, greatly lowering operational complexity and improving sample recovery.
Product Overview
Ultra-high-throughput LysN-directed digestion samples feature low per-well input, large sample numbers, and pronounced matrix variability. These characteristics pose specific challenges to LysN digestion and desalting workflows:
1. Cumulative Loss under Low Input
At the 384-well scale, per-well sample amounts are extremely limited. In traditional stepwise workflows, repeated transfers and tube opening more readily cause adsorption and residual loss, making low-abundance N-terminal-related peptides difficult to retain consistently.
2. Inter-Well Condition Inconsistency
Differences in salt concentration, buffer systems, and detergent backgrounds among samples are amplified under ultra-high-throughput conditions. Traditional methods struggle to unify the reaction environment, leading to fluctuations in LysN digestion kinetics.
3. Amplified Batch Operation Errors
As the number of wells increases, pipetting deviations, insufficient mixing, or differences in reaction time windows are more likely to accumulate into systematic variability in traditional workflows, compromising large-scale data comparability.
4. Difficulty Balancing Cleanup and Recovery
Insufficient desalting can cause ion suppression and elevated background signals, whereas excessive cleanup may reduce peptide recovery. This trade-off is particularly pronounced under low-input and high-throughput conditions.
Advantages
✅ 384-Well Ultra-High-Throughput Parallel Processing
The MtoZ Biolabs' 384-well C-trap LysN digestion and desalting kit supports one-time processing of large batches of samples, reduces repetitive manual operations, and is better suited for screening-based and multi-condition parallel experiments.
✅ More Stable LysN-Directed Digestion
The system is optimized around the characteristic of LysN cleaving at the N-terminus of lysine residues, enabling more consistent cleavage output under complex and low-input backgrounds.
✅ Integrated In-Plate Workflow
Digestion and desalting are performed sequentially within the same plate, reducing intermediate transfers and significantly lowering sample loss and operator-induced errors.
✅ Integrated Desalting for Improved MS Stability
The workflow simultaneously attenuates the impact of salts and interfering components, reduces ion suppression, and helps maintain stable LC-MS/MS performance in large-scale analyses.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
