The MtoZ Biolabs Trace Protein Extraction Kit is designed for extremely limited and highly valuable low-input samples, enabling highly efficient protein recovery and facilitating proteomic analysis of needle-biopsy tissues and small cell aggregates.
Product Overview
Low-input samples obtained from laser microdissection, needle biopsy, or single-cell research are exceptionally valuable; however, conventional protein extraction approaches are often unsuitable for such materials because:
1. Extremely Limited Input
Samples may contain only a small number of cells or microgram-scale tissue. Protein recovery using standard workflows can be markedly reduced, or even undetectable, owing to adsorption to vessel surfaces and losses incurred during sample transfer.
2. Pronounced Non-specific Adsorption
At microscale volumes, proteins readily adsorb to centrifuge tube walls and pipette-tip surfaces, leading to irreversible sample loss.
3. Loss Introduced by Procedural Steps
In traditional multi-step extraction and purification workflows, each step can leave residual material. For low-input samples, cumulative losses can be critical.
4. High Sensitivity Requirements for Downstream Analyses
The recovered trace proteins must be compatible with ultra-sensitive analytical platforms (e.g., single-cell mass spectrometry and ultra-sensitive Western blotting).
Advantages
1. Highly Efficient Recovery
The MtoZ Biolabs Trace Protein Extraction Kit employs an ultra-concentrated lysis formulation together with proprietary purification materials to maximize binding and elution of trace proteins, enabling efficient recovery from microgram- and even nanogram-level inputs.
2. Ultra-Low Adsorption Across the Workflow
The kit includes specialized low-adsorption centrifuge tubes and purification columns to minimize surface adsorption and reduce sample loss.
3. "Single-Tube" Streamlined Operation
The entire extraction and purification procedure can be performed sequentially within a single tube, eliminating cumbersome transfers and thereby reducing residue and loss associated with transfer steps.
4. Compatibility with Ultra-Sensitive Detection
The final elution volume is small and yields a high protein concentration; the buffer system is clean and is well suited for ultra-sensitive BCA quantification, Western blotting, and next-generation mass spectrometry platforms.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
