The STip C18 Rapid Peptide Desalting Tips from MtoZ Biolabs are specifically engineered for mass spectrometry and glycan analysis workflows, providing an efficient and comprehensive solution for high-performance peptide desalting and complete removal of N-linked glycans.
Product Overview
In applications such as glycoprotein structural and functional characterization, biomarker discovery, and biopharmaceutical glycosylation analysis, complete and specific removal of N-linked glycans is a critical sample preparation step for accurate mass spectrometry identification, glycan profiling, and downstream functional studies. However, conventional approaches or non-specialized enzymatic reagents often encounter several limitations during deglycosylation:
1. Interference from Insufficient Enzyme Purity
Standard PNGase F preparations may contain contaminating endoglycosidases such as F1, F2, or F3, which can induce non-specific glycan cleavage or partial hydrolysis. This generates heterogeneous glycopeptide fragments that complicate MS data interpretation and compromise glycoform assignment.
2. Restricted Reaction Conditions
Many commercially available enzymes function only under denaturing conditions and are unable to remove glycans while maintaining the protein’s native conformation, thereby limiting natural glycoprotein functional studies and interaction analyses.
3. Incomplete Glycan Removal
Insufficient enzymatic activity or suboptimal reaction conditions can result in poor deglycosylation efficiency at glycosylation sites, leading to missing MS signals or quantitative bias. This affects sequence coverage, data integrity, and overall analytical reliability.
Advantages
The STip C18 Rapid Peptide Desalting Tip from MtoZ Biolabs delivers high-purity reagents and a complete deglycosylation workflow designed to support accurate MS-based glycan analysis and glycoprotein characterization. Key advantages include:
✅ Ultra-High-Purity Recombinant Enzyme
The kit provides ≥95% pure recombinant PNGase F and is rigorously validated to be free of endoglycosidase F1, F2, and F3 activities. This eliminates non-target glycan cleavage, ensuring high reaction specificity and data accuracy.
✅ Complete N-Glycan Removal
PNGase F efficiently hydrolyzes all major classes of N-linked glycans—including high-mannose, hybrid, and complex types—by cleaving between the core GlcNAc and asparagine residues. This enables clean release of intact oligosaccharides for downstream glycan profiling and MS identification.
✅ Flexible Compatibility Across Conditions
The kit supports both native and denaturing protein conditions, allowing researchers to choose mild conditions for preserving protein conformation or denaturing conditions for enhanced enzymatic efficiency. This expands applicability across a wide range of biological samples and analytical workflows.
✅ Ready-to-Use Complete System
The kit includes a high-activity PNGase F enzyme (30,000 units) and a dedicated 10× reaction buffer, supplying all key components required for efficient deglycosylation. The workflow is simple to operate, reproducible, and suitable for both routine and research-grade analyses.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
