The Soil Protein Extraction Kit from MtoZ Biolabs is designed for complex soil matrices, addressing key obstacles such as humic-acid interference and low microbial biomass, while enabling efficient recovery of proteins for environmental metaproteomic analyses.
Product Overview
Soil is a critical sample type in metaproteomics; however, its high compositional complexity and abundant co-extracted interferents present substantial challenges for protein extraction:
1. Severe Interference from Organic Matter (E.G., Humic Acids)
Soil is rich in humic acids that share similar physicochemical properties with proteins, making them prone to co-extraction. These compounds can strongly inhibit downstream proteolytic digestion and mass spectrometry–based analyses and represent a major bottleneck in soil protein studies.
2. Low Protein Abundance and Strong Adsorption
Microbial biomass in environmental samples is often low, and proteins can be strongly adsorbed onto mineral particles, leading to poor extraction efficiency and yields that may be insufficient for downstream detection.
3. High Risk of Protein Degradation
Active microbial enzyme systems in soil, together with repeated freeze–thaw cycles, may cause degradation of target proteins prior to extraction.
4. Strong Sample Heterogeneity
Soils from different sources (e.g., agricultural soils, forest soils, and extreme environments) can differ substantially in composition, requiring extraction methods with high robustness and broad applicability.
Advantages
✅ Efficient Removal of Humic Acids
The MtoZ Biolabs Soil Protein Extraction Kit incorporates dedicated precipitation and washing steps to efficiently remove organic interferents such as humic acids. The resulting protein preparations exhibit markedly improved purity and are suitable for direct mass spectrometry–based analysis.
✅ Strong Desorption and Efficient Lysis
The optimized lysis buffer facilitates release of proteins adsorbed on soil particles and promotes effective microbial cell lysis, thereby improving overall protein recovery.
✅ Rapid Inhibition of Proteolysis to Protect Targets
The kit includes broad-spectrum protease inhibitors that rapidly suppress soil protease activity at the initial stage of extraction, helping preserve protein integrity.
✅ Broad Compatibility with Environmental Samples
The workflow has been rigorously evaluated using multiple representative soil types (e.g., varying pH and organic-matter content) and demonstrates stable and strong extraction performance, supporting environmental metaproteomics applications.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
