The MS-compatible Magnetic Kit (Protein A/G) from MtoZ Biolabs is specifically engineered to provide high-performance, low-background immunoprecipitation (IP) for mass spectrometry-based proteomics.
Product Overview
Conventional immunoprecipitation methods often face major limitations during functional protein studies or interactome analyses:
1. Antibody Compatibility Constraints
Traditional Protein A or Protein G alone exhibits substantial variability in binding efficiency across antibody species and subclasses. This frequently results in suboptimal enrichment, especially when working with rare or precious antibodies.
2. Non-Specific Binding Interference
Agarose or magnetic beads commonly show non-specific protein adsorption, producing high background signals that obscure true interactors during mass spectrometry analysis.
3. Incompatible Elution Conditions
Standard low-pH elution buffers efficiently release antigens but also cause heavy- and light-chain antibody fragments to detach, severely complicating downstream MS identification.
4. Insufficient Sensitivity
Limited capture efficiency for low-abundance targets can make it difficult to meet the sensitivity requirements of advanced mass spectrometry workflows.
Advantages
The MtoZ Biolabs' MS-compatible Magnetic Kit (Protein A/G) offers several superior features that overcome the shortcomings of conventional IP approaches:
1. Exceptional Antibody Compatibility
Utilizing a recombinant Protein A/G fusion, the kit efficiently binds all IgG subclasses from multiple species, including human, mouse, rat, and rabbit, substantially increasing antibody utilization and enrichment reliability.
2. Ultra-Low Background
Specially treated agarose beads feature an optimized surface coating that minimizes non-specific protein adsorption, ensuring high-purity samples and improved confidence in MS data.
3. Flexible Elution Options
Both acidic elution and competitive mild elution are provided. Acidic elution is ideal for Western blotting, while the gentle competitive elution preserves antibody integrity, making it perfectly suited for mass spectrometry workflows.
4. High-Sensitivity Detection
The optimized binding buffer system enhances capture efficiency for low-abundance targets. Combined with high-resolution mass spectrometry, the kit enables robust protein identification even from limited or low-input samples.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
