Biotin Magnetic Beads

In modern life science research, the separation and purification of biomacromolecules (such as proteins and nucleic acids) are fundamental prerequisites for downstream studies. With the advancement of molecular biology, immunology, and proteomics, magnetic separation technology has become widely applied due to its advantages of ease of operation, high efficiency, and strong specificity. Among them, magnetic nanoparticles developed based on the biotin-avidin action mechanism, namely biotin magnetic beads, have become an ideal tool in the fields of immunoprecipitation, protein interaction research, nucleic acid capture and cell sorting.

  

MtoZ Biolabs focuses on the development and supply of protein sample preparation and magnetic bead consumables, and has launched high-performance biotin magnetic beads to provide researchers with efficient and reliable laboratory consumables, significantly improving experimental reproducibility and data quality.

  

Product Overview

MtoZ Biolabs' biotin magnetic beads are composed of superparamagnetic nanoparticles covalently coupled with high-purity biotin, with a particle diameter of approximately 200 nm. These beads offer an exceptionally large specific surface area and excellent magnetic responsiveness. They can efficiently and specifically bind to biomolecules conjugated with streptavidin or avidin (such as antibodies, proteins, peptides, nucleic acids, etc.), enabling effective separation, enrichment, and purification of target molecules.

  

Product specifications include 200 μL, 1 mL, and 5 mL packaging options to meet various needs, from small-scale experiments to high-throughput automated screening. Each milligram of magnetic beads can bind ≥150 pmol of streptavidin, offering high binding capacity and broad applicability.

  

Protocol

1. Magnetic Bead Pre-treatment

(1) Recommended volume: Use 20 μL of magnetic bead suspension (containing 200 μg of beads) per sample; adjust the amount according to the concentration and volume of the target molecule.

(2) Resuspension and washing: Gently pipette the beads to fully resuspend. Place the tube on a magnetic rack for 1 minute to separate, then discard the supernatant.

(3) Washing steps: Add 500 μL of 1× TBS or an appropriate binding buffer. Repeat the wash 2-3 times, separating magnetically for 1 minute each time to remove impurities.

  

2. Sample Binding

(1) Incubate the magnetic beads with biotin-labeled ligands (antibodies, proteins, peptides, etc.) in buffer (20 mM potassium phosphate buffer, 0.15 M NaCl, pH 7.5) at 25 °C for 30 min, or at 4 °C for 1 h.

(2) After incubation, wash the beads three times with buffer.

(3) Elute the bound sample with an appropriate elution buffer (6 M guanidine hydrochloride).

  

Features and Benefits

1. High Binding Capacity and Rapid Reaction

(1) ≥150 pmol of streptavidin can be bound per mg of magnetic beads.

(2) Nanoscale particle structure enhances binding kinetics, reduces handling time, and preserves sample activity.

  

2. High Specificity and Low Background

(1) Covalently conjugated biotin ensures specific binding to streptavidin-linked molecules.

(2) Low non-specific adsorption results in higher purity and more reliable outcomes.

  

3. Broad Compatibility and Flexible Applications

(1) Suitable for proteins, antibodies, nucleic acids, and other sample types.

(2) Compatible with various experimental setups such as immunoprecipitation, cell sorting, and pull-down assays.

  

4. Easy Handling and Automation-Ready

(1) Centrifugation-free with streamlined steps.

(2) Adaptable to automated high-throughput screening platforms, improving experimental efficiency.

  

5. Safe and Environmentally Friendly Design

(1) Glycerol-free and mass spectrometry-compatible.

(2) Stable at pH 6-8 and safe to operate at room temperature.

  

Applications

MtoZ Biolabs' biotin magnetic beads are widely applicable in the following research and application areas:

1. Immunoprecipitation (IP/Co-IP)

Enrich target antigens using streptavidin-conjugated antibodies to capture protein complexes.

  

2. Nucleic Acid-Protein Interaction Studies (DNA/RNA Pull-Down)

Isolate proteins bound to specific nucleic acid probes to elucidate regulatory networks.

  

3. Cell Surface Marker Sorting

Perform immunomagnetic separation of labeled cells to support flow cytometric analysis.

  

4. Biomarker Capture and Enrichment

Efficiently enrich target proteins or nucleic acids from biological fluids to enhance detection sensitivity.

  

5. Hybridization Assays and Signal Amplification

Support signal enhancement in methods such as Southern/Northern blot and ELISA.