The Glu-C Protease Digestion Kit launched by MtoZ Biolabs is specifically designed for glutamic acid residue C-terminal specific cleavage, ideal for the controlled digestion of structurally complex proteins, aiding in mass spectrometry-based proteomics sample preparation and analysis.
Product Overview
Glu-C protease cleaves specifically at the C-terminal of glutamic acid residues, making it especially suitable for structurally complex and stable protein samples. The C-terminal cleavage of glutamic acid depends on the exposure of its site and the protein's folding state. The following challenges are encountered during this process:
1. Limited Exposure of Glutamic Acid Sites
Due to the folding structure of some proteins, glutamic acid residues may be in a hidden state, making them difficult to cleave effectively under standard conditions.
2. Differences in Sample Types
Proteins from different sources can vary in structure and composition, which may affect digestion efficiency and consistency, leading to incomplete digestion or biases.
3. Low Digestion Efficiency
The folding and stability of proteins significantly impact enzyme activity. Some samples may exhibit uneven digestion or partial cleavage of proteins.
4. High Sensitivity to Experimental Conditions
The digestion process is highly sensitive to changes in experimental conditions, and slight variations may affect the consistency of digestion and the reproducibility of results.
Advantages
✅ Specific Cleavage
The kit is designed for C-terminal specific cleavage of glutamic acid residues, ensuring effective recognition and cleavage of target peptides in complex samples.
✅ Optimized Reaction System
The optimized reaction conditions enhance the cleavability of glutamic acid sites, improving digestion efficiency and stability.
✅ Wide Adaptability
MtoZ Biolabs' Glu-C protease digestion kit is compatible with a wide range of protein samples, ensuring stability and reproducibility during the digestion process.
✅ Good Reproducibility and Stability
Standardized digestion conditions minimize the impact of operational variations, supporting consistency across different batches of samples.
✅ Compatible with Downstream Analysis
The digested peptides can be directly used for LC-MS/MS, Western blot, immunoenrichment validation, and other downstream analysis platforms.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
