MtoZ Biolabs's Asp-N Protease Digestion Kit is specifically designed for the N-terminal cleavage of aspartic acid residues, enabling precise and efficient cleavage of target proteins. It is widely used in mass spectrometry analysis and post-translational modification studies.
Product Overview
The N-terminal cleavage of aspartic acid residues relies on the degree of site exposure, and differences in the protein samples' origin and background add challenges to digestion consistency and stability.
1. Site Exposure Variability
Due to different protein folding states, some aspartic acid sites are difficult to expose, resulting in incomplete digestion and affecting peptide coverage.
2. Sample Source Variability
Samples from different origins (e.g., cells or tissues) vary significantly in concentration, impurity background, and buffer systems, adding complexity to the digestion process.
3. Digestion Consistency Issues
Differences in protein stability and structure affect digestion efficiency, leading to inconsistencies between batches and reducing experimental comparability.
4. High Control Requirements
The digestion process is sensitive to variations in conditions, with slight reaction fluctuations causing differences in digestion efficiency, affecting result consistency.
Advantages
✅ Precise Cleavage
The kit is specifically designed for N-terminal cleavage of aspartic acid residues, ensuring high specificity and obtaining structurally consistent peptides.
✅ Optimized Reaction System
Optimized reaction conditions enhance the cleavability of aspartic acid sites, ensuring consistency and stability in the digestion process.
✅ Broad Compatibility
MtoZ Biolabs' Asp-N protease digestion kit is suitable for samples from various sources, ensuring stable digestion and good reproducibility.
✅ Stability and Reproducibility
Standardized digestion conditions minimize the impact of operational fluctuations, ensuring consistency of results between batches.
✅ Strong Downstream Compatibility
The enriched peptides can be directly used for liquid chromatography-tandem mass spectrometry (LC-MS/MS), supporting subsequent protein identification and quantitative analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
