The E.coli HCP(Clone Strain) Polyclonal Antibody from MtoZ Biolabs is a broad-coverage anti-HCP reagent developed for sensitive detection of residual E.coli host cell proteins (HCP) from a defined production strain to support process monitoring and product purity assessment.
Product Overview
E. coli is a widely used host for recombinant protein production, but endogenous E.coli host cell proteins can persist through lysis and downstream purification and may remain as residual impurities. Common challenges in detecting these impurities include:
1. Insufficient Coverage
Single antibodies may not detect all residual E. coli host cell proteins, especially those present at low abundance or strain-specific, leading to incomplete or inaccurate measurements of impurity levels.
2. Non-Specific Binding
Complex sample matrices and high product concentrations can cause non-specific binding, resulting in increased background noise and reduced assay sensitivity, making it harder to accurately assess HCP levels.
3. Lot Variability
Inconsistent antibody performance across lots can complicate trending and cross batch comparisons.
4. Limited Method Flexibility
Some reagents may not perform well with common analytical techniques such as Western blot or immunoassays, limiting the versatility of the method.
Advantages
✔️Strain Matched Recognition
MtoZ Biolabs' E.coli HCP(Clone Strain) Polyclonal Antibody is raised against clone strain HCP profiles to improve relevance for your expression system and strengthen detection confidence.
✔️Broad Polyclonal Coverage
Multi-epitope binding supports wider HCP recognition, including diverse proteins across a range of sizes and properties.
✔️Cleaner, More Specific Readout
Optimized reactivity helps reduce background and improve interpretability in complex samples.
✔️Consistent Lot Performance
Manufactured under controlled conditions with lot-level QC to support reproducible performance, making trending and cross-batch comparisons more dependable.
✔️Flexible Downstream Compatibility
Validated for common readouts such as Western blot and dot blot, and suitable as a robust reagent for immunoassay development workflows where method transferability matters.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
