MtoZ Biolabs' CHO Host Cell Proteins Polyclonal Antibody is a highly specific antibody designed to detect and quantify residual Chinese hamster ovary (CHO) host cell proteins (HCPs) in recombinant biologics, ensuring the purity of biopharmaceutical products and compliance with regulatory standards.
Product Overview
The detection of residual HCPs in CHO cell-derived therapeutics is challenging due to the complexity of the host cell proteins and the interference from high product concentrations during purification. Common challenges include:
1. Limited Sensitivity
Residual CHO HCPs can persist at trace levels after purification, and low-intensity signals are easy to miss or fall below the assay detection range. This increases the risk of underreporting residuals, especially when you need confident trending across process steps or lots.
2. Non-Specific Binding
High concentrations of the target biologic, along with formulation components (salts, detergents, excipients), can elevate background and promote nonspecific binding. The result is reduced signal-to-noise, false positives, or masked HCP signals that make quantitation less reliable.
3. Batch-to-Batch Variability
If antibody reactivity shifts between lots, the same sample can yield different readouts over time. This complicates longitudinal monitoring, cross-batch comparisons, and method transfer, and it can trigger extra verification work during QC and validation.
4. Incompatibility with Certain Downstream Assays
Limited compatibility can force workflow changes, extra optimization, or parallel reagents to support different readouts and development stages.
Advantages
✔️Comprehensive HCP Coverage
MtoZ Biolabs' CHO Host Cell Proteins Polyclonal Antibody is specifically developed to detect a broad range of CHO HCPs, including low-abundance proteins, addressing the challenge of incomplete or partial detection and ensuring accurate quantification of residual HCPs
✔️Reduced Non-Specific Binding
Optimized to minimize non-specific binding, this polyclonal antibody ensures a clean signal even when dealing with complex matrices or high product concentrations, resulting in improved accuracy and lower background interference.
✔️Consistent Performance Across Lots
Each batch of this antibody undergoes rigorous quality control to guarantee reliable and reproducible results across different lots, addressing the problem of batch-to-batch variability and enabling easier data trending and comparison.
✔️Broad Compatibility with Assays
The CHO Host Cell Proteins Polyclonal Antibody is compatible with a variety of downstream assays, including Western blot, ELISA, and other immunoassays, making it an ideal choice for consistent and flexible use in different experimental workflows.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
